THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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Ê (t) c ¿ o- o 170 Figure 28. Serr¡n oPL concentrations in sampres obtalned from four ewes at 6-h intervals over a 4å day period from day t20_125 of gestati on . 2pm 8pm 2om 8qm Zprr 8pm 2äm 8om Zp. gprn Zj, aå. Zp* gp.n 2..r, gcrn 2pm gprn TIME
Sheep no. l{6 25í 960 464 5?0 680 ?90 I 110 9 110 10 ll0 lr u0 12 110 t3 110 14 ll.0 lá 110 16 110 17 tn tE 130 19 lfn 20 130 2r t32 171 TABLE IV Comparison of matemal and fet¡l serum opl, concent¡ations Days of M¡t¿mal (M) oPL gê¡tåtio! (n¡lnl) 2.5 18 13 t?5 700 670 l90 7m 590 160 220 290 410 315 ?00 320 5d) u0 Fetal (F) oPL (ng,/l¡l) Fet¡ts | 2 (Moan) n ,¡{t 66 76 123 12A 95 l{t r32 18 63 88 t77 54 51 72 49 70 69 6l 67 75 64 ã9 1t 44 66 95 82 68 60 50 5{) 50 40 30 141 .t9 94 s8 7L 123 95 t3? 70 135 5:' 8t 8l 6t 7l 77 64 59 6E 76 7l ùb 50 35 24 , _- : I "r: il---;;¡d-;:.ii4 R¡tio of F:M 16.6 3.9 13.7 ' 4.1 t0.5 0.3 0.08 0.12 0.32 0.10 0.13 0..t o.22 0.20 0.r8 0.19 0.08 0,16 0.06 o.22
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THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
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LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
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NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
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6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
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-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
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-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
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109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
Page 141 and 142: (2) Assav of ìuteo.trgpic effec.t
Page 143 and 144: EXPERIMENT # TI (7 days after HCG)
Page 145 and 146: 123 to progesterone (l/300 djlution
Page 147 and 148: SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150: 127 Fractioñâtión of oPL. from c
Page 151 and 152: 129 Figure 6. carborymethyì(cM,)-c
Page 153 and 154: t 3't óo0 ,oo 800 900 FRACIION r,t
Page 155 and 156: E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158: TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160: 137 Figure lì. A, Poìyacrylamide
Page 161 and 162: Leaf blank to correct nr.rmbering
Page 163 and 164: 14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166: o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168: ¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170: HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172: Tissue used for binding ôtudies Li
Page 173 and 174: Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176: ¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178: P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180: o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182: F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184: ! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186: 163 Figure 24 shows the sensltivity
Page 187 and 188: 165 D. STUDIES ON THE SECRETION OF
Page 189 and 190: E .Dc À o - art & 167 Flgure 26. P
Page 191: lJhen blood samples were taken four
Page 195 and 196: 't73 fetal cotylêdons at various d
Page 197 and 198: TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200: l. Súrglcat Method 't77 Figure 3l
Page 201 and 202: = >€ ,3 ; I 179 rwt 76-0450 o----
Page 203 and 204: dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206: ! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208: l ol
Page 209 and 210: I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212: l89 Flgure 37. Percentage ot l2sl-o
Page 213 and 214: 19t column is shown in Figure 3BA.
Page 215 and 216: - 193 3H -couNdro _________SPECtFtC
Page 217 and 218: TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220: z o 197 Figure 40, Radioreceptor as
Page 221 and 222: 5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224: SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226: Ch aracteri zati on 203 Analytical
Page 227 and 228: 205 size, corresponding to the mole
Page 229 and 230: 207 the specific binding of oPL to
Page 231 and 232: 209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
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221 The nature of thelarge nolecula
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223 data argue for a close interact
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]. TABLE OF CHEMICAL PROPERTIES GUI
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cor,t coAT (bPL) (cpl) 3. TABLE OF
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À) E 229 Figure 43. The secretion
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231 H. POSSIBLE ROLT OF OVINE PLACE
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233 Our studies on the blnding of o
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235 through its binding to cotpus l
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237 Furthermore, oPL neasurement in
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239 circulationand alìantoic f'lul
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SECTICN IX PUBLICATIONS A) Manuscr
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SECTION X: BIBLIOGRAPHY Abramovich,
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Beas,F., Saìinas, 4., GonzalezrF.,
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247 Brody,S,, and Carlstrom,G.,J.Cl
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249 Crosignanì ,P.G. , Nencioni ,
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25t Friesen,H.G., Endocrinoìogy 76
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253 Grinwich,D.L., M. Hichens, and
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25s Hum, V.G., Knipfel , J.G., and
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?57 Knopp,R,H,, H,J. Ruder,E. Herra
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259 Manku,M.S., and D.F. Horrobin,
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261 , :iir .,.-;;r-,:, Morgan, F.J.
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263 Pierce,G.B., Midþley,A.R. and
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?65 Shepard, T.H.., Gen. Comp. Endo
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267 Tsuruhara,T., Dufau,M.L., Hickm
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