B) Abstracts: 242 1. Chan,J,S.D., Kel'ly, p., D.Carr, and H.G. Friesen, elinical Research XXII, 703A,197b 2. Carr,D., Chan,J.S.D., Hoìdaway, I.M., and H.G. Friesen, Cl inica'l Research XXII,A34A,lg7s 3. Chan,J.S.D., and H.G. Friesen, Texas Reports on Bioìogy and Medicine, 33,1976 4. ihan,J.S.D., Robertson,H.A., and H,G. Friesen, in: Proc. of s8th Meeting of the Endocrine Soclety, san Francisco, Cal. ll3A,.l976 5. Carnegie, J.A., Chan,J.S.D., Robertson,H.A., Friesen,H.G., and M.E. McCul ly, in 69th Annual Meeting of Amerícan Society of AnÍmal Sc.ience, July,l977 6. Tay1or,M.J., Jenkin,G., Chan,J.S.D., Friesen,H.G., Thorburn, D,G., and J.S. Robinson, in t.53rd Meeting of the Society for Endocrinoìogy, May, Engtand, l97g
SECTI<strong>ON</strong> X: BIBLIOGRAPHY Abramovich,D.R. , and Rowe,p.,J.Endocr. E6:62.1 ,1973 Abramovich,D.R.,Baker,T.G., and NeaJ,p.,J.Endocr. 60:129,1974 Adcock,E.ld.,III, Teasdale,F., Agust,C.S.,Cox,S.,Meschìa,G.,Battaglia,F.C., and Naughton, M.A. ,science l8l :945,1973 Agate, F.J.,Jr., Amer. J. Anat. 90..257,1952 Aiabor,L.N., and Yen,S.S.:C.,Am. J. Obstet. Gynecoì. ll2:908,1972 Albert,A. and I. Derner, J. Clin Endocrinol Metab Z0:ì225,.l966 Albert,4., J. Clin. Endocrinol . Metab. 29:.l504,'l969 Albert,A.,Underdahì,1 .U., Greene,L.F., and Lorenz, N. Endocrinology 9l:72g,ì91 AloJ's.M., Ede'lhoch, H,, Handwerger, S., and Sherwood,L,M. tndocrinoìogy9r: 728,1972 243 Aìlen, l{.R., and Moore, R.M., J.Reprod.Fert. 29:313,1972 Amoroso, E.C., In Marshal't 's physìoìogy of Reproduction. 3rd Ed., Ed. A.S. parks, Longmars Green,London,lg52 An¡¡roso, E.C. , Br. Med. Bul'l . ll:1.l7,.l955 AmoRoso, E,C., and C.A. Finn in Zuckerman, S.,(ed)., The Ovary, Vo.l . I, Acad. Press, N,y. , p45l,.l962 Amenomori ., Y., Chen,C.L., and Meites,J., Endocrinology 86:506,1970 Anc!rews, P., Biochem. J. lll:799,1969 Arslan, 14., wolf,R.c., and Meyer, R.K,.in proc Soc. Exp. Biot(N.y.) l2s:349,tse Ars'lan, M., Wolf,R.C., and Meyer,R.K.,in prc. 2nd Ann. Meetlng of the Soxiety for the Study of Reproduction.Univ. of Calif.,Davis, calìf. (Abstract), p3,1969
- Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
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LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
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NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
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6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
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-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
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-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
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109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
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EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
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SECTION V : RESULTS (r) LACTOGEN DU
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127 Fractioñâtión of oPL. from c
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129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
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TABLE I TABLE OF PURIFICATION OF OV
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137 Figure lì. A, Poìyacrylamide
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Leaf blank to correct nr.rmbering
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14r Anal.vtical Sodi um Dodecyl Sql
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o 9 x F- -)- 9 rU I æ -t f () LT'
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¿ o s : t45 Figure 15b. Displaceme
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HoR MoNE coNc€NrRArto ln ß/mt )
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Tissue used for binding ôtudies Li
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Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
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¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
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P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
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o c o U o òs -o c2o ao eÀo o 'o .
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F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
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! o 't6 l HORMO¡¡ E COr{CENr FAIt
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163 Figure 24 shows the sensltivity
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165 D. STUDIES ON THE SECRETION OF
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E .Dc À o - art & 167 Flgure 26. P
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lJhen blood samples were taken four
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Sheep no. l{6 25í 960 464 5?0 680
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't73 fetal cotylêdons at various d
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TABLE VI OPI CONCENTRATTON IN FETAL
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l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
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dUrlng PrêOnancy in thè Ewes tBr
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! ñ' l83 NoRMON! C oNCENTßArlO þ
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l ol
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I I I "+ _t rl \l :- 'i rl -l l87 a
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l89 Flgure 37. Percentage ot l2sl-o
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- Page 215 and 216: - 193 3H -couNdro _________SPECtFtC
- Page 217 and 218: TABLE IX /- BIOASSAY OF qROIItIfH p
- Page 219 and 220: z o 197 Figure 40, Radioreceptor as
- Page 221 and 222: 5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
- Page 223 and 224: SFCT.IoN II : pl.scussr0N 4d ã 1È
- Page 225 and 226: Ch aracteri zati on 203 Analytical
- Page 227 and 228: 205 size, corresponding to the mole
- Page 229 and 230: 207 the specific binding of oPL to
- Page 231 and 232: 209 than with prolactin-like effect
- Page 233 and 234: 2t1 the jncrease in oPRL binding si
- Page 235 and 236: 213 The resuìts obtained indicate
- Page 237 and 238: 215 on the role af PL in the fetus.
- Page 239 and 240: Surgi ca1 method- 217 Our studies o
- Page 241 and 242: 219 5.Relationships amonq opRL. oGH
- Page 243 and 244: 221 The nature of thelarge nolecula
- Page 245 and 246: 223 data argue for a close interact
- Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
- Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
- Page 251 and 252: À) E 229 Figure 43. The secretion
- Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
- Page 255 and 256: 233 Our studies on the blnding of o
- Page 257 and 258: 235 through its binding to cotpus l
- Page 259 and 260: 237 Furthermore, oPL neasurement in
- Page 261 and 262: 239 circulationand alìantoic f'lul
- Page 263: SECTICN IX PUBLICATIONS A) Manuscr
- Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
- Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
- Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
- Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
- Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
- Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
- Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
- Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
- Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
- Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
- Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
- Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm