THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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262 Ni swender,G.D., Roche,J. F,, Foster,D.L., and Midgley,A. R.,Jr., proc. Soc. Exp. Biol. Med. 129:901 ,1968 Nisu1a,B.C,,Koh1er,P.0., Vaitukaitis, J.L., Hershman,J.M., and Ross,G,T., J.Clin. tndocr. Metab. 37:664,1973 Nixon,l,ll.E., Tuììner, t,.l.Ì^l., Rayford, P.L., and Ross, G.T., Endocrinology 88:702 ,l 971 Nixon, Vl.E., Hodgen,G.D., Niemann,l.l.H., Ross,G.T. and Tül lner,l,l.l^J., tndocri nol ogy,l971 Odeìl,1,J.D., Bates,R. , R.W., Rivl.in,R.S. , Ljpsett,M,B., and Hertz,R. , J. C'lin. Endocr. Metab. 23:658,ì963 0esterlìng,M.J., Cox,S.E., and Carrington,E.R., Am.J. C.lin. Nutr. 30:,lg2,.1977 0ppenheímer,J.H,, Sqúef ,R.,Surks,M. I., and Haner,H. J.Cl in, Invest,42: I 769 ,l 963 Orczyk,G.P., Hichens,M., Arth,G.E., and Behrman,H.R. " in progesterone, Methods of Hormone Raciioinmunoassay, ed. B.M. tlaffe and H.R. Behrman, Acad. press. New york, pp347,1g74 Papkoff,H., Biochenr. Biophys. Res. Commun. 58:397,1974 Parlow, 4.F., and Ward, 0.N., Hum. pituitary Gonadotroplns,p204,l96ì Par1ow,A.F., Danne,T.A., and Dignam,l^l.J.,J. Cl.in Endocr. Metab. 31 r213,1970 Patrito,L.C., Fì ury,A. ,Rosato ,J . ,and Martin,A., Hoppe-Seylerrs Z Phys iol. Chem. 354: l'129,1973 Pattillo,R.A., Shalaby,M.R., Hussa,R.0., Bahl,0.p., and MattinglV,R.F., Obstet. Gnecoi . 47:557 ,1976 Pearse,W,H., and Kaiman,H.,Am.J. Obstet. Gynecol . 9g:572,1g67 Pencharz,R.I. and J.A. Long,Am J Anat 53;1..l933 Pencharz,R.I. and l,l.R.Lyons,proc. Soc. Exp. Biol. Med. 3l:ìì3.l ,.l934 Penny,R., 0lambluvonnu, N.D.,and Fraiser,S.D., pediatrics 53:4j,.1974 Peters,J.P.,Mann,E.B., and Heinemann,M., yaìe J. Biol . Med.Z0:449,ì948 Plcard,c., .oms,H.A.,Barasse,E., and conard,v., Diabetotogia 4: r6,.r96g
263 Pierce,G.B., Midþley,A.R. and Beals,T.F., Lab. Invest, l3:451 ,t964 Pierce,J.G. , Bahì ,0. p. ,Corne l ì ,J . S. , and SwamÍnathan,N.,J.Bioì. Chem. 246:2321 ,1971 Purti lo,D.T.,Hallgren,H.l'l.,and yunis,t.J., Lancet 1:769,1972 Pugsley,L.J. ,Endocrinology 39: I6l ,1946 Rahnan,S.A ",Hingoramr',V.,and Laumas,K. R.,J. Reprod. Fert. 38:228,197 4 Rathnarn,P., and Saxena,B.B.,in Gonadotropins 0varjan Dev., proc. l,lorkshop f4eet., ì969,pj44,j972 Ray'E.hl. ' s.c- Averil l,l,.l.R.Lyons and R.E, Johnson, Endocrinology 56:359,r955 Rayford, P.1., Va i tu ka i ti s ,J . L . , Ross,G.T. , Morgan,F.J., and Canfield,R.E., Endocrino'l ogy 91 :144,1972 Deddy,S., and hl.B. l,,latkins,J.Endocrino.logy 6S:.l85,1975 Rees,L,H., Burke,C.lll., Chard,T., Evans,S.l^1., and Letchworth,A.T., Nature (London ) 254 :620,1 97 s Reìd,R.1., and N.T. Hinks,Austr.J Agric. Res. l3:1092,1962a Reìd,R.1., and N.T. Hinks,Austr. J. Agric. Res. l3:llì2,ì962b Rejsfeld,R.A.,U.J. Lewis and D.E.t,,li lliams,Nature 195:281,1972 Riggi,Endocrinol ogy 79 : 709,1 960 Rizkallah,T. " GLrrpide,E. , and van de t¡leile,R.L. ,,J. Clin.Enclocr. l4etab. 29:92,.1969 Rochr.r¡ir ,ii., Varnavides,L.A",anrl llawle-,,p,R.,J. Enclocrinoi fi:4A7,i9lZ Robertson,li"A. and 1.,R. Sarda"J. Enclocr. 49:4A7 1971 Roberts.rn,l1.C. and H.G. Friesen, Endocrìnoìogy ,lg7S Rolschau, J., Date, J., Kristoffersen,K., pedersen,G.T., and U.ì rich,M., Acta 0bstet. Gynecol . Scand. 54:34.l ,.l975 Ross,G. T., Va jtukaitj s,J.L., and Robbins,J.B., jn Structure_Actìvìty ireiat jonsh j ps of prote jn and polypept.ìcie Hornlooes,,,pì53,ì 972
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
Page 11 and 12:
LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
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NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
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6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
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-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
Page 33 and 34:
-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
Page 81 and 82:
(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
Page 113 and 114:
9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
Page 121 and 122:
Char4ctêf ization Pfocedures l. Ch
Page 123 and 124:
t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
Page 127 and 128:
AFsa)¡- ProceiiureS 105 Specific b
Page 129 and 130:
107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
Page 143 and 144:
EXPERIMENT # TI (7 days after HCG)
Page 145 and 146:
123 to progesterone (l/300 djlution
Page 147 and 148:
SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150:
127 Fractioñâtión of oPL. from c
Page 151 and 152:
129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158:
TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160:
137 Figure lì. A, Poìyacrylamide
Page 161 and 162:
Leaf blank to correct nr.rmbering
Page 163 and 164:
14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166:
o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168:
¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170:
HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172:
Tissue used for binding ôtudies Li
Page 173 and 174:
Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176:
¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178:
P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182:
F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184:
! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186:
163 Figure 24 shows the sensltivity
Page 187 and 188:
165 D. STUDIES ON THE SECRETION OF
Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
Page 191 and 192:
lJhen blood samples were taken four
Page 193 and 194:
Sheep no. l{6 25í 960 464 5?0 680
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't73 fetal cotylêdons at various d
Page 197 and 198:
TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200:
l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
Page 203 and 204:
dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206:
! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208:
l ol
Page 209 and 210:
I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212:
l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
Page 215 and 216:
- 193 3H -couNdro _________SPECtFtC
Page 217 and 218:
TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220:
z o 197 Figure 40, Radioreceptor as
Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224:
SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226:
Ch aracteri zati on 203 Analytical
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205 size, corresponding to the mole
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207 the specific binding of oPL to
Page 231 and 232:
209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252: À) E 229 Figure 43. The secretion
Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256: 233 Our studies on the blnding of o
Page 257 and 258: 235 through its binding to cotpus l
Page 259 and 260: 237 Furthermore, oPL neasurement in
Page 261 and 262: 239 circulationand alìantoic f'lul
Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
Page 283: 261 , :iir .,.-;;r-,:, Morgan, F.J.
Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm
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