THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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258 Li,c.H., Dixon,J.S., and D. Chung, Science lZ3:56,197.l Li,C.H., Proc Amer phi'l Soc.,ll6:365,.l962 Li,c.H., Ann. sclavo l2;6sl ,l97o Li, C.H., Lactogenic Horm., Ciba Found. Symp. , l9l1 ,p7_ZZ Li,C.H., Dixon,J.S., Lo, T.8., pankov, y.A., and Schmidt,K.D., Na ture (L ondon )224:695 ,.1969 Li,C.H.,Dixon,J.S., Li.T.B., Schmidt,K.D., and pankov,y.A., Arch. Biochem. Biophys. l4l:705,.l970 Li,C.H., Dixon,J.S., Gordon,D., and Knorr,,l., Int. J. pept. protein Res. 4:151 ,.l972 Li,C.H., Dixon,J.S., and Chung,D., Arch. Biochinr.Biophys. 155:95,.l 973 Lin,T.M., and Haibert, S.p.,Sclence 193:1249,1976 Lin,T.M,, Halbert,S.p., and Spel.lacy,[^,.N.,J. Cì in lNvest. 34:576,1914 Lín'T.M,, Haibert,S.P., Spellacy,!J.N., and Gall,S., Afi. J.obstet. Gynecol . 124:382,1967a Lin,T.l,l., Ha'lbert,S.p., and Kiefer,D., J. Clin. Invest. 57:466,ì976b Lindsay,D.B., proc Nutri Soc. 30:272,.197.1 Loke,Y.l,l., and pepys,M.B., Am. J.0bstet. Gyneco.l .tZl:37,.l975 Lopez-Quijada,C. , and Blazquez,E., Life Scï. part II:25,197.l Louvet,J.I., Hannan,S.M., Nisu,la,B.C., Ross,G.T., Birken,S., and Canfield,R., Endocrinol ogy 99 :1126,197 6 Lowry, 0.H., N.,l . Rosebrough,A.L. Farr, R.J. Randaì1, J.Biol . Chem.l93:265,.l91 Lunenfeld,8., and Eshkol ,A., Vit. Honn. (N.y.)25:.l37,1967 Li,thtlh:l: l¡¡ut:.ha.*Êgi olgz ig|,r szo Lyons, no.R., R.E. Johnson,R.D,Cole and C.H. Li in Smith,R.W., 0.H.Gaebler, and C.N.Long(eds), The Hypophysea,l Growth l{ormone, Nature and Ac*ons, McGraw-Hi.l'l . New york, p461 ,ì9S5 Madruzza,G., Rlv ltal . Ginec 6:173,1927
259 Manku,M.S., and D.F. Horrobin, ,J.Int. Res. Commun I i16,1973 Manns, J.G,, and Boda,J.M., Endocrinology 76:ì109,.l965 Mann,L. I., Lutz,M.,Schu lman,H., and Romney,S.C.,Am. J. Obstet. Gynecoì. 98:.l151 ,.l972 Martin,J.14., and Friesen,H.G., Endocrinology 84:6.l9,1969 Martal ,J., and J. Djiane, Cell Tlss. Res. lB4:427,.l977 Martal ,J., and J. Djiane, J. Reprod Fert. 49:285,.|972 Martal,J. and ,1 . Djlane. Biochem. Blophys. Res. Commun. 63:770,ì975 Maruo,T.,Endocr. Jpn. 23:65,.l976 Maruo,T.,Asitaka,Y.,Moch izuchi,M., and Tojo,S., Endocr. Jpn. 2l : 499,1 974 Mason,N. R., Schaffer, R.J., and Toomey,R.,Endocr.i no'l ogy 93 : 34, t 973 MclJilI iams,D., Cal 1ahan,R.C., and Boime,I.,proc. Nat'l . Acad. Sci. USA, 7 4:1024 ,1977 McNeÍ11y,4., and H.G. Friesen, Endocr.ino'logy,l97B Matthies,D.L. , Anat. Rec. 151 r583,1965 Matthies,D.L., Anat. Rec.'154:384,ì966 MatthÌes,D.L., Anat. Rec. .l59:55,t967 Matthies,D.L., and l^1,R. Lyons, Proc. Soc. Exp. Biol . Med. .l36:520,197ì Matthies, D.L., In Lacotgenic Hormones, Fetal Nutrition, and Lactation, p291 ,1974 Mayer, G, and R. Canivene, C.R. Soc. Bio'l (paris) 144:4t0,.l950 Menon,K.M.J., BÌochem.Biophys. Res. Conxnun. 56:363,'1974 Merz,W.E,, Hil genfeldt,U., Dorner,M., and Brossmer,R., Hoppe-Seyìer' s Physiol . Chem. 355:1035,1974a Merz,hl.E., Hiìgenfeldt, U. o Brossner,R., and Rehberger, G., Hoppe-Setler's Physiol . Chem. 355:'1046,1974b
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
Page 3 and 4:
To My parents and wife: Mn & Mrs. K
Page 5 and 6:
CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
Page 11 and 12:
LIST OF FIGURES FIGURE PAGE I Linea
Page 13 and 14:
22 Radioreceptor assay for oPRL us.
Page 15 and 16:
Effect of PGF,. and lactogenic horm
Page 17 and 18:
ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
Page 25 and 26:
NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
Page 27 and 28:
6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
Page 29 and 30:
-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
Page 33 and 34:
-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
Page 47 and 48:
-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
Page 51 and 52:
-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
Page 59 and 60:
-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
Page 81 and 82:
(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
Page 121 and 122:
Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
Page 125 and 126:
103 B : MFTÍÍOD OF DETECTION AND
Page 127 and 128:
AFsa)¡- ProceiiureS 105 Specific b
Page 129 and 130:
107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
Page 137 and 138:
l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
Page 141 and 142:
(2) Assav of ìuteo.trgpic effec.t
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EXPERIMENT # TI (7 days after HCG)
Page 145 and 146:
123 to progesterone (l/300 djlution
Page 147 and 148:
SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150:
127 Fractioñâtión of oPL. from c
Page 151 and 152:
129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158:
TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160:
137 Figure lì. A, Poìyacrylamide
Page 161 and 162:
Leaf blank to correct nr.rmbering
Page 163 and 164:
14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166:
o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168:
¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170:
HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172:
Tissue used for binding ôtudies Li
Page 173 and 174:
Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176:
¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178:
P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182:
F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184:
! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186:
163 Figure 24 shows the sensltivity
Page 187 and 188:
165 D. STUDIES ON THE SECRETION OF
Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
Page 191 and 192:
lJhen blood samples were taken four
Page 193 and 194:
Sheep no. l{6 25í 960 464 5?0 680
Page 195 and 196:
't73 fetal cotylêdons at various d
Page 197 and 198:
TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200:
l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
Page 203 and 204:
dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206:
! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208:
l ol
Page 209 and 210:
I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212:
l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
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- 193 3H -couNdro _________SPECtFtC
Page 217 and 218:
TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220:
z o 197 Figure 40, Radioreceptor as
Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224:
SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226:
Ch aracteri zati on 203 Analytical
Page 227 and 228:
205 size, corresponding to the mole
Page 229 and 230: 207 the specific binding of oPL to
Page 231 and 232: 209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252: À) E 229 Figure 43. The secretion
Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256: 233 Our studies on the blnding of o
Page 257 and 258: 235 through its binding to cotpus l
Page 259 and 260: 237 Furthermore, oPL neasurement in
Page 261 and 262: 239 circulationand alìantoic f'lul
Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
Page 279: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm
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