THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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1. POSSIBLE EXPERIMENTS TO DEMONSTRATE THT BIOLOGICAL EFFTCTS OF
OVINE PLACENTAL LACTOGTN IN SHEEP
ì. Manrmotrop ic tffect:
The mammotropjc effect of opl in sheep may be
demonstrated by the effects of opL on lactogenesis in explant
cultures of mammary gland tissues from early pregnant ewes
according to the method described by Jeulin-Bailly et al (1973)
and Collier et al(1977). If the availability of ovine placental
lactogen permlts, one may examine the effects of opL on the
norphology of the ovine mamnary gìands after chronic adninistration
,.ú:: ;'--l
of oPL into the ewes which have primed with sterojds. The methodology
wiìl be similar to that described by Beck(t972).
2. Metabolic Effect:
The metabolÍc effects of opL may be demonstrated using
in vitro and in vivo systems. The effect of opL on lipolysis and
incorporation of amino acid could be studied using the method
described by Friesen(1965), Turile et al(i966), Riggi(ì966),
Genazzani et al (.1969), except that ovine tissues are used instead
of those from rat or rabbit. .
If the avaìlabilîty of opl permits, opl may be
infused into early pregnant ewes for a short period in order to
observe the effects of opl on the levels of several netaboJites "
such as FFA; g'lucase, and amino nitrogen ln the clrculailon.