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Macroalgae and phytoplankton as indicators of ... - Naturstyrelsen

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tial variation would give a better spatial description <strong>and</strong> reduce the r<strong>and</strong>om<br />

variation between sites.<br />

Coupling algal variables to water quality<br />

The variation in water quality variables w<strong>as</strong> initially analysed using a<br />

model similar to the algal model. The model describes water quality<br />

variables with respect to area-specific variation, site-specific variation,<br />

se<strong>as</strong>onal variation <strong>and</strong> year-to-year variation among hydrological years,<br />

i.e. July-June. For each water quality variable we calculated area-specific<br />

marginal means.<br />

Algal variables were related to physico-chemical variables through multiple<br />

regression analysis using backward elimination. First we introduced<br />

all the potential independent variables in the regression, <strong>and</strong> then excluded<br />

variables one by one until only the significant variables remained.<br />

The analyses were conducted on a spatial b<strong>as</strong>is to explain differences in<br />

algal parameters between various co<strong>as</strong>tal are<strong>as</strong>/sub-are<strong>as</strong>.<br />

Testing the "Spanish index" on Danish data<br />

A test <strong>of</strong> the "Spanish index" on Danish data dem<strong>and</strong>s some adjustment<br />

since Danish <strong>and</strong> Spanish algal data are collected differently <strong>and</strong> species<br />

composition <strong>and</strong> depth distribution differ. A first adjustment regards the<br />

definition <strong>of</strong> the three components <strong>of</strong> the index which also affects the<br />

data range <strong>and</strong> thus the scoring system. However, the principle <strong>of</strong> the<br />

scoring system, i.e. its depth <strong>and</strong> area dependence may also need adjustment<br />

for the index to be applicable under Danish conditions.<br />

Our approach for testing the "Spanish index" is the following:<br />

• Verify whether the individual components <strong>of</strong> the index reflect nutrient<br />

gradients in Danish co<strong>as</strong>tal waters since this is a prerequisite for<br />

including them in the index.<br />

• Identify whether each component is depth dependent <strong>and</strong> whether its<br />

level varies between are<strong>as</strong>, e.g. depending on salinity. This part <strong>of</strong> the<br />

test will tell us whether the principle <strong>of</strong> the Spanish scoring system<br />

can be transferred to Danish conditions.<br />

Below we explain how the "Spanish index" is defined <strong>and</strong> translated to<br />

Danish conditions <strong>and</strong> how the scoring system <strong>of</strong> the "Spanish index"<br />

operates.<br />

Definition <strong>of</strong> the three components <strong>of</strong> the "Spanish index"<br />

The first component <strong>of</strong> the index is the cover <strong>of</strong> characteristic species. In<br />

Spain, this variable is <strong>as</strong>sessed by estimating the percentage <strong>of</strong> the stable<br />

substratum <strong>of</strong> the sample area which is covered by 'characteristic species'<br />

<strong>as</strong> defined for Spanish are<strong>as</strong>. Characteristic species are those which are<br />

not opportunistic. We translate the Spanish 'characteristic species' to the<br />

Danish 'late-successional species'. The first component <strong>of</strong> the Spanish index<br />

is therefore approached by the Danish variable 'cumulated cover <strong>of</strong><br />

late-successional species'. The data range is 1-100% for the Spanish variable<br />

but may exceed 100% for the Danish variable.<br />

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