Abstract Book of EAVLD2012 - eavld congress 2012

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S4 - P - 12 COMPARISON OF THE EFFICIENCY OF DIFFERENT PARASITOLOGICAL DIAGNOSTIC METHODS USED IN ANALYSIS OF DEHYDRATED SEWAGE SLUDGES Jolanta Zdybel, Tomasz Cencek, Jacek Karamon National Veterinary Research Institute, Departament of Parasitology and Invasive Disease, Pulawy, Poland Ascaris, Toxocara, Trichuris, eggs, sewage sludge, fertilizers Introduction The use of sewage sludge as fertilizer is connected with microbiological and parasitological hazards in terms of public health safety. Therefore, it is necessary to carry on proper parasitological investigations of this type of samples. Majority, of current parasitological diagnostic procedures using in sewage sludge surveys are derived from soil samples investigation. This kind of procedure are characterized by low efficiency, due to occurrence of polyelectrolytes in sewage sludge samples. Therefore, new method of sewage sludge parasitological diagnostics was developed. The methods is focused on detection of parasitic nematodes eggs, belonging to Ascaris, Trichuris and Toxocara genus. It is combination of floatation and sedimentation method, preceded by sample prolonged dispersion. The purpose of this study is to compare the efficiency of the method developed in Department of Parasitology and Invasive Diseases to other methods which are used in laboratories worldwide. References 1. Zdybel J., Karamon J., Cencek C.: The occurrence of eggs of parasitic roundworms (genera: Ascaris, Trichuris, Toxocara) in organic and mineral-organic fertilizers and in sewage sludge. Zycie Wet.2009,84(12),992-996. 2. Simonart T., Roussel S., Gireaudot-Liepman M.F.: desk study on European standard for the enumeration of diable helminth ova in sludge, soil and solid waste. HORIZONTAL-WP3-5, France 2003. Materials & methods For this study we used randomly chosen 10 dehydrated sewage sludge samples derived from Polish waste water treatment plants. Each sample weighed 10 grams (dry weight) and was examined by our own method, PN-Z-19000-4 method, flotation method described by Quinn, method according to Environmental Protection Agency (EPA) and Triple flotation (TF) method according to ANFOR XP X33-017 . Microscopy (X20) was used to estimate the number of nematode eggs. Results Results concerning percentage of positive samples and numbers of detected parasite eggs obtained with the use of different methods were presented in Table 1. Table. 1. Mean number of parasite eggs and % of positive samples detected in sewage sludges samples with the use of 5 different methods. Methods Mean number of detected eggs (% of positive samples) Ascaris Toxocara Trichuris Quinn 2.2 (70%) 2.1 (90%) 0.3 (30%) PN-Z-19000-4 0.3 (40%) 0.15 (30%) 0 Triple flotation 0.6 (10% 3.3 (40%) 0 EPA 1.7 (80%) 1.3 (100%) 1.3 (20%) Own method 16.4 (100%) 11.1 (100%) 1.7 (100%) Discussion & conclusions The results of the present study demonstrated that method developed in Department of Parasitology and Invasive Diseases is six-fold more efficient than flotation method devised by Quinn, seven-fold more efficient than TF method and EPA method, 65- fold more efficient then PN-Z-19000-4 method. The adaptation of described method in other parasitological laboratories is highly recommended.
List of Abstracts (in order of number) S1-K-01 VAN DER POEL WIM H. M. S1-K-02 SCHWARZ STEFAN S1-O-01 BOSS CHRISTINA S1-O-02 STRUTZBERG- MINDER KATRIN S1-O-03 BOUWSTRA RUTH S1-O-04 GÓRNA KAMILA S1-O-05 NARDINI ROBERTO S1-O-06 BALLAGI ANDREA S1-O-07 VAN MAANEN KEES S1-O-08 REVILLA- FERNÁNDEZ SANDRA S1-O-09 COOLEY WILLIAM S1-O-10 LANGEVELD JAN S1-O-11 REBORDOSA- TRIGUEROS XAVIER S1-O-12 O'NEILL RONAN S1-O-13 SCHIRRMEIER HORST S1-O-14 BOSS CHRISTINA S1-O-15 PORQUET-GARANTO LOURDES S1-O-15 VILLA ALEIDA S1-O-17 BALLAGI ANDREA S1-O-18 LOPEZ PABLO S2-K-01 SOLDAN ANDREW S2-O-01 NORTH SARAH THE EMERGENCE OF SCHMALLENBERG VIRUS – HOW TO RESPOND TO NEW EPIZOOTICS IN EUROPE ASSESSING THE ANTIMICROBIAL SUSCEPTIBILITY OF BACTERIA OBTAINED FROM ANIMALS ORAL FLUIDS – SAMPLE MATRIX FOR EFFECTIVE HERD HEALTH MONITORING DIAGNOSTIC PROPERTIES OF SEVERAL PRRS ANTIBODY ELISAS IN THE CONTEXT OF A LONGITUDINALSTUDY OF A FARM USING DIFFERENT VACCINATION STRATEGIES INTRODUCTION RATE OF A LOW PATHOGENIC AVIAN INFLUENZA VIRUS INFECTION IN DIFFERENT DUTCH POULTRY SECTORS A DUPLEX ONE-STEP REAL TIME RT-PCR FOR DIAGNOSIS OF FOOT AND MOUTH DISEASE VALIDATION OF A COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES ANTI-P26 OF EQUINE INFECTIOUS ANEMIA VIRUS IN EQUINE SERA DIAGNOSTIC PERFORMANCE OF A COMMERCIAL PRRS SERUM ANTIBODY ELISA ADAPTED TO ORAL FLUID SPECIMENS: FIELD SAMPLES SUBTYPING OF SWINE INFLUENZA VIRUSES BY MULTIPLEX REAL-TIME PCR IMPORTANCE OF CONTINUOUS VALIDATION OF MOLECULAR METHODS FOR ROUTINE DIAGNOSIS OF PRRSV RNA IN CLINICAL SAMPLES VIRAL DIAGNOSIS USING TRANSMISSION ELECTRON MICROSCOPY A BEAD BASED MULTIPLEX IMMUNOFLUOROMETRIC ASSAY FOR SCREENING AND CONFIRMATION OF ALL MAJOR PRION TYPES IN SHEEP USING ORAL FLUID FOR THE SEROLOGICAL MONITORIZATION OF PRRSV CIRCULATION IN A GROUP OF INFECTED GILTS ENHANCED DETECTION OF BOVINE RESPIRATORY VIRUSES BY INCLUSION OF COHORT ANIMALS THE FIRST YEAR OF OBLIGATORY BVD CONTROL IN GERMANY – DIAGNOSTIC STRATEGIES, RESULTS AND EXPERIENCES EUROPEAN PRRSV – DIAGNOSTIC SOLUTIONS FOR A RAPIDLY MUTATING VIRUS SEROLOGICAL ANALYSIS AND MONITORING OF IBR IS IT POSSIBLE TO CONTROL IBRGE ANTIBODIES IN A BULK TANK MILK? DEVELOPMENT AND VALIDATION OF A REAL-TIME PCR ZEN GEL MIX FOR THE DIAGNOSIS AND QUANTIFICATION OF COXIELLA BURNETII RING TEST EVALUATION FOR THE DETECTION OF PRRSV ANTIBODIES IN ORAL FLUID SPECIMENS USING A COMMERCIAL PRRSV SERUM ANTIBODY ELISA REAL TIME PCR, MYCOPLASMA GALLISEPTICUM, MYCOPLASMA SYNOVIAE, MYCOPLASMA MELEAGRIDIS WHEN DO YOU WANT THE RESULT? HOW MUCH DO YOU WANT TO PAY! DEVELOPMENT OF A RAPID ISOTHERMAL ASSAY TO DETECT TAYLORELLA EQUIGENITALIS, THE CAUSATIVE AGENT OF CONTAGIOUS EQUINE METRITIS
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2 nd CONGRESSOFTHEEUROPEAN ASSOCIAT
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Welcome Dear colleagues, Welcome to
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worked with many diagnostic compani
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Products and services for scientist
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16:4518.15 20:0023:00 [S1.]Oralpres
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Day4 Wednesday,4July2012 4 th sessi
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Oral presentations “General Sessi
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antigens or attenuated vaccines can
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conditions, e.g. Staphylococcus aur
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S1 - O - 1 ORAL FLUIDS - SAMPLE MAT
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S1 - O - 03 INTRODUCTION RATE OF A
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S1 - O -05 VALIDATION OF A COMPETIT
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S1 - O - 07 SUBTYPING OF SWINE INFL
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S1 - O - 09 VIRAL DIAGNOSIS USING T
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S1 - O - 11 USING ORAL FLUID FOR TH
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S1 - O - 13 THE FIRST YEAR OF OBLIG
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S1 - O - 15 SEROLOGICAL ANALYSIS AN
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S1 - O - 17 RING TEST EVALUATION FO
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Oral presentations “Diagnostics a
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S2 - O - 01 DEVELOPMENT OF A RAPID
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S2 - O - 03 EVALUATION OF RAPID HRS
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Oral presentations “Emerging, re-
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advances, the fundamental tools of
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S3 - O - 02 25 YEARS OF PASSIVE SUR
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S3 - O - 04 EQUINE NOCARDIOFORM PLA
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S3 - O - 06 DETECTION OF SCHMALLENB
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S3 - O - 08 SCHMALLENBERGVIRUS: SER
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S3 - O - 10 DIAGNOSTIC ASPECTS OF S
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Oral presentations “New technique
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acid and organic solvent. Then a dr
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S4 - O - 02 BIOLOG GENERATION III,
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S4 - O - 04 MATRIX-ASSISTED LASER D
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S4 - O - 06 15 MINUTE ELISA USING A
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S1 - P - 01 LAWSONIA INTRACELLULARI
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S1 - P - 03 DETECTION OF PRRSV ANTI
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S1 - P - 05 DEVELOPMENT AND EVALUAT
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S1 - P - 07 ANALYTICAL EVALUATION O
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S1 - P - 09 PTS FOR BVDV ANTIGEN DE
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S1 - P - 11 ANTIMICROBIAL SUSCEPTIB
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S1 - P - 13 IDENTIFICATION OF GLOBI
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S1 - P - 15 ACCURACY OF PARATUBERCU
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S1 - P - 17 VIROLOGICAL SURVEILLANC
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S1 - P - 19 CHARACTERIZATION OF EXT
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S1- P - 21 ANALYSIS OF THE EFFICIEN
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S1 - P - 23 RELIABLE AND EARLY DETE
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S1 - P - 25 GENOTYPIC VARIABILITY D
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S1 - P - 27 PROFICIENCY TESTING (PT
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S1 - P - 29 LOW PATHOGENIC AVIAN IN
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S1 - P - 31 PRRSV DIAGNOSTICS BY RT
Page 101 and 102: S1 - P - 33 APPLICATION OF A HERD P
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Page 121 and 122: S3 - P - 08 EMERGENCE OF SCHMALLENB
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Page 131 and 132: S3 - P - 18 PREVALENCE OF COXIELLA
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Page 155 and 156: S1-P-03 BALLAGI ANDREA S1-P-04 BENI
Page 157 and 158: S3-P-06 KELLER SELINA S3-P-07 KÖNI
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Abstract Book of EAVLD2012 - eavld congress 2012

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