S3 - O - 11 PRELIMINARY VALIDATION OF THE ID SCREEN ® SCHMALLENBERG VIRUS INDIRECT ELISA P. Pourquier, 1 , C. Loic, 1 , S. Zientara, 2 , E. Breard, 2 , C. Sailleau, 2 , C. Viarouge, 2 , A.-B.Cay, 3 , N. De Regge, 3 1 IDvet, Montpellier, France 2 ANSES Maisons-Alfort, France Introduction Schmallenberg virus (SBV) is the name given to a vectortransmitted orthobunyavirus related to Shamonda virus, initially reported in November 2011 to cause foetal congenital malformations and stillbirths in cattle, sheep, and goats. The virus has since been detected in Germany, the Netherlands, Belgium, France, Luxembourg, Italy, Spain and the United Kingdom. Serological testing is essential in order to study and control this new emerging disease. While SBV specific antibodies can be detected by virus neutralization tests and indirect immun<strong>of</strong>luoresence, these techniques are time-consuming, not suitable for high-throughput, and do not <strong>of</strong>fer standardized result interpretation. Available as <strong>of</strong> March <strong>2012</strong>, the ID Screen ® Schmallenberg virus Indirect ELISA is the first ELISA developed for SBV diagnosis. The test allows for the detection <strong>of</strong> SBV antibodies in ruminant serum and plasma. It is a rapid, standardized assay which is automatable and therefore suited to high throughput testing. This study presents validation data for this test. Data will be added for any second generation tests developed by IDvet between March and June <strong>2012</strong>. Material & methods - The ID Screen ® Schmallenberg virus Indirect ELISA was performed as per manufacturer’s specifications. - Specificity was studied using panels <strong>of</strong> sera collected prior to 2010. - Sensitivity was evaluated on sera tested positive with the virus neutralization test (VNT). - An experimental infection was performed in April <strong>2012</strong> in order to evaluate the seroconversion response in cattle, sheep and goats. Results & Discussion Preliminary validation studies indicate excellent test specificity and high correlation with other serological techniques, making the the ID Screen ® Schmallenberg virus Indirect ELISA an efficient tool for disease surveillance and epidemiological studies. 3 CODA-CERVA, Department <strong>of</strong> Viral Diseases, Brussels, Belgium Schmallenberg virus, serology, ELISA
Oral presentations “New techniques in bacteriology, parasitology and pathology” (4 th session)
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