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Welcome to the 31st IUBS General Assembly and Conference on ...

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that <str<strong>on</strong>g>the</str<strong>on</strong>g> Chinese samples originated from <str<strong>on</strong>g>the</str<strong>on</strong>g> USA. A<br />

genetic diversity gradient was observed from <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

sampling localities in China: sites from <str<strong>on</strong>g>the</str<strong>on</strong>g> lower Yangtze<br />

River showed <str<strong>on</strong>g>the</str<strong>on</strong>g> most genetic diversity with 2<br />

co‐occurring haplotypes, while <strong>on</strong>ly a single haplotype<br />

was observed in some sites far from <str<strong>on</strong>g>the</str<strong>on</strong>g> estuarine of<br />

Yangtze River. This biogeographic pattern was c<strong>on</strong>sistent<br />

with an anecdotal report that Chinese samples of P. Clarkii<br />

were first introduced in<str<strong>on</strong>g>to</str<strong>on</strong>g> Nanjing <str<strong>on</strong>g>and</str<strong>on</strong>g> neighboring cities<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> lower Yangtze River by <str<strong>on</strong>g>the</str<strong>on</strong>g> Japanese army during<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> World War II. Future sampling from Japan will provide<br />

more detailed genetic informati<strong>on</strong> about col<strong>on</strong>izati<strong>on</strong><br />

his<str<strong>on</strong>g>to</str<strong>on</strong>g>ry of P. Clarkiiam<strong>on</strong>g China, Japan <str<strong>on</strong>g>and</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> USA.<br />

Populati<strong>on</strong> genetic diversity <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

demographic his<str<strong>on</strong>g>to</str<strong>on</strong>g>ry of two freshwater crabs<br />

(Sinopotam<strong>on</strong> acutum<str<strong>on</strong>g>and</str<strong>on</strong>g>S.shensiense)<br />

endemic <str<strong>on</strong>g>to</str<strong>on</strong>g> China<br />

F FANG, HY SUN,YK JI, Q ZHAO<str<strong>on</strong>g>and</str<strong>on</strong>g>W GAO<br />

freshwater crabs experienced rapid populati<strong>on</strong><br />

expansi<strong>on</strong> since 0.1 Ma, corresp<strong>on</strong>ding <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> warm <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

humid climate after <str<strong>on</strong>g>the</str<strong>on</strong>g> retreat of <str<strong>on</strong>g>the</str<strong>on</strong>g> last extensive glacial<br />

period (0.5‐0.175 Ma). We calculated significant<br />

am<strong>on</strong>g‐populati<strong>on</strong> structure for both species (S. acutum:<br />

Φ ST =0.919, P< 0.001; S.shensiense: Φ ST =0.707, P< 0.001).<br />

In c<strong>on</strong>clusi<strong>on</strong>, climate fluctuati<strong>on</strong>s may have played<br />

important roles for genetic diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> populati<strong>on</strong><br />

his<str<strong>on</strong>g>to</str<strong>on</strong>g>ry of S. acutum<str<strong>on</strong>g>and</str<strong>on</strong>g>S. shensiense. Additi<strong>on</strong>ally, <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

present habitat loss <str<strong>on</strong>g>and</str<strong>on</strong>g> fragmentati<strong>on</strong> in distributi<strong>on</strong><br />

range could make influence <strong>on</strong> diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> distributi<strong>on</strong><br />

of <str<strong>on</strong>g>the</str<strong>on</strong>g>se 2 freshwater crabs. Special protecti<strong>on</strong> measures<br />

should be taken because of <str<strong>on</strong>g>the</str<strong>on</strong>g>ir genetic uniqueness <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

preserve <str<strong>on</strong>g>the</str<strong>on</strong>g>ir genetic diversity.<br />

Populati<strong>on</strong> genetic diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> genetic<br />

structure of an endemic true freshwater<br />

crabSinopotam<strong>on</strong> h<strong>on</strong>anense (Decapoda:<br />

Brachyura) in China based <strong>on</strong> microsatellite<br />

DNA analysis<br />

Jiangsu Key Labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry for Biodiversity <str<strong>on</strong>g>and</str<strong>on</strong>g> Biotechnology,<br />

College of Life Sciences, Nanjing Normal University, Nanjing<br />

210046, China. Email: sunh<strong>on</strong>gying@njnu.edu.cn. This work<br />

was supported by grant from <str<strong>on</strong>g>the</str<strong>on</strong>g> Nati<strong>on</strong>al Natural Science<br />

Foundati<strong>on</strong> of China (31071902) <str<strong>on</strong>g>to</str<strong>on</strong>g> HYS.<br />

Pleis<str<strong>on</strong>g>to</str<strong>on</strong>g>cene climatic fluctuati<strong>on</strong>s have played major roles<br />

in shaping <str<strong>on</strong>g>the</str<strong>on</strong>g> genetic diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> populati<strong>on</strong> his<str<strong>on</strong>g>to</str<strong>on</strong>g>ry<br />

observed in extant species in China. However, patterns of<br />

diversificati<strong>on</strong> are species idiosyncratic <str<strong>on</strong>g>and</str<strong>on</strong>g> indicative of<br />

Zhu LIN <str<strong>on</strong>g>and</str<strong>on</strong>g> H<strong>on</strong>gying SUN<br />

Jiangsu Key Labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry for Biodiversity <str<strong>on</strong>g>and</str<strong>on</strong>g> Biotechnology,<br />

College of Life Sciences, Nanjing Normal University, Nanjing<br />

210046, Jiangsu, China. This work was supported by grants<br />

from <str<strong>on</strong>g>the</str<strong>on</strong>g> Nati<strong>on</strong>al Natural Science Foundati<strong>on</strong> of China<br />

(31071902) <str<strong>on</strong>g>to</str<strong>on</strong>g> HYS, <str<strong>on</strong>g>and</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Priority Academic Program<br />

Development of Jiangsu Higher Educati<strong>on</strong> Instituti<strong>on</strong>s<br />

(PAPD).Email: sunh<strong>on</strong>gying@njnu.edu.cn<br />

variati<strong>on</strong>s in geographic divergence.<br />

The genetic diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> genetic structure of an endemic<br />

Sinopotam<strong>on</strong>acutum<str<strong>on</strong>g>and</str<strong>on</strong>g>S. shensiense are true freshwater<br />

Chinese freshwater crab, Sinopotam<strong>on</strong>h<strong>on</strong>anense, were<br />

crabs endemic <str<strong>on</strong>g>to</str<strong>on</strong>g> China, both of which have a sympatric<br />

investigated by analyzing 9 microsatellite loci for 485<br />

distributi<strong>on</strong> al<strong>on</strong>g <str<strong>on</strong>g>the</str<strong>on</strong>g> Qingling–Daba Mountains. We<br />

samples collected from 28 localities across current<br />

combined maternally inherited mtDNA <str<strong>on</strong>g>and</str<strong>on</strong>g> biparentally<br />

distributi<strong>on</strong> range of species. Basic statistics showed that<br />

inherited microsatellite DNA <str<strong>on</strong>g>to</str<strong>on</strong>g> explore <str<strong>on</strong>g>the</str<strong>on</strong>g> genetic<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> mean overall values of H O <str<strong>on</strong>g>and</str<strong>on</strong>g> H E were<br />

diversity <str<strong>on</strong>g>and</str<strong>on</strong>g> demographic his<str<strong>on</strong>g>to</str<strong>on</strong>g>ry of S.<br />

0.6125±0.2250 <str<strong>on</strong>g>and</str<strong>on</strong>g> 0.6402±0.1538, respectively. The<br />

acutum<str<strong>on</strong>g>and</str<strong>on</strong>g>S.shensiense, respectively, in order <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

populati<strong>on</strong> genetic diversity declines linearly with<br />

underst<str<strong>on</strong>g>and</str<strong>on</strong>g> how freshwater crabs located in m<strong>on</strong>tane<br />

increasing latitude (r 2 =0.415, P

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