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Welcome to the 31st IUBS General Assembly and Conference on ...

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Plants are used in <str<strong>on</strong>g>the</str<strong>on</strong>g> modern era for <str<strong>on</strong>g>the</str<strong>on</strong>g> extracti<strong>on</strong> <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

development of drugs <str<strong>on</strong>g>and</str<strong>on</strong>g> a large number of patients rely<br />

<strong>on</strong> medicinal herbs. Jatropha tanjorensisisis a herbaceous<br />

plant of Euphorbiaceae family <str<strong>on</strong>g>and</str<strong>on</strong>g> it shows<br />

intermediacy in phenotypic characters between<br />

J.gossypifolia<str<strong>on</strong>g>and</str<strong>on</strong>g> J.curcas. It is comm<strong>on</strong>ly called hospital<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g>o far, catholic vegetable, iyana‐ipaja <str<strong>on</strong>g>and</str<strong>on</strong>g> lapalapa. It is<br />

used for fencing, as an edible leafy vegetable <str<strong>on</strong>g>and</str<strong>on</strong>g> as<br />

medicine.<br />

Tank‐raised Clarias gariepinus (mean <str<strong>on</strong>g>to</str<strong>on</strong>g>tal length 28.39 ±<br />

0.33 cm SE; mean weight, 99.34 ± 2.48g SE) were<br />

obtained were obtained locally from a commercial fish<br />

farm <str<strong>on</strong>g>and</str<strong>on</strong>g> transferred <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Animal <str<strong>on</strong>g>and</str<strong>on</strong>g> Envir<strong>on</strong>mental<br />

Biology Research Labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry, Delta State University,<br />

Abraka where <str<strong>on</strong>g>the</str<strong>on</strong>g>y were held in large plastic aquaria of<br />

140L capacity with clean borehole water. They were <str<strong>on</strong>g>the</str<strong>on</strong>g>n<br />

acclimatized for 14 days, during which time <str<strong>on</strong>g>the</str<strong>on</strong>g>y were fed<br />

with commercial fish feed pellets (Coppens) twice daily.<br />

Fresh leaves of J. tanjorensis were obtained locally <strong>on</strong><br />

campus. They were air‐dried for 2 weeks <str<strong>on</strong>g>and</str<strong>on</strong>g> later<br />

oven‐dried for 3 hours, after which <str<strong>on</strong>g>the</str<strong>on</strong>g>y were ground <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

fine powder <str<strong>on</strong>g>and</str<strong>on</strong>g> dissolved in distilled water. The soluti<strong>on</strong><br />

was evaporated <str<strong>on</strong>g>to</str<strong>on</strong>g> dryness using a rotary evapora<str<strong>on</strong>g>to</str<strong>on</strong>g>r.<br />

Five test c<strong>on</strong>centrati<strong>on</strong>s (2.0, 4.0, 6.0, 8.0 <str<strong>on</strong>g>and</str<strong>on</strong>g> 10.0g/L)<br />

were prepared by serial diluti<strong>on</strong> for injecti<strong>on</strong> in<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

fish. 2mL of <str<strong>on</strong>g>the</str<strong>on</strong>g> soluti<strong>on</strong> was injected intramuscularly <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> fish. Blood samples were obtained from <str<strong>on</strong>g>the</str<strong>on</strong>g> caudal<br />

circulati<strong>on</strong> after 96hours <str<strong>on</strong>g>and</str<strong>on</strong>g> used for <str<strong>on</strong>g>the</str<strong>on</strong>g> measurement<br />

of haema<str<strong>on</strong>g>to</str<strong>on</strong>g>crit, haemoglobin c<strong>on</strong>centrati<strong>on</strong><str<strong>on</strong>g>and</str<strong>on</strong>g> red blood<br />

cell counts. Empirical data of <str<strong>on</strong>g>the</str<strong>on</strong>g> results obtained were<br />

subjected <str<strong>on</strong>g>to</str<strong>on</strong>g> statistical analysis using <strong>on</strong>e‐way analysis of<br />

variance (ANOVA) <str<strong>on</strong>g>to</str<strong>on</strong>g> test for level of significance between<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> various c<strong>on</strong>centrati<strong>on</strong>s of J. tanjorensis injected<br />

intramuscularly.<br />

The haema<str<strong>on</strong>g>to</str<strong>on</strong>g>logical values of haemoglobin (HB),<br />

haema<str<strong>on</strong>g>to</str<strong>on</strong>g>crit (Hct), <str<strong>on</strong>g>and</str<strong>on</strong>g> red blood cell (RBC) in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

injected fish were significantly lower (P< 0.05) than <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

c<strong>on</strong>trol while <str<strong>on</strong>g>the</str<strong>on</strong>g> Mean Corpuscular Volume (MCV), Mean<br />

Corpuscular Haemoglobin (MCH) <str<strong>on</strong>g>and</str<strong>on</strong>g> Mean Corpuscular<br />

Haemoglobin c<strong>on</strong>centrati<strong>on</strong> (MCHC) were significantly<br />

higher (P< 0.05) in <str<strong>on</strong>g>the</str<strong>on</strong>g> injected fish than <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>trol.<br />

This study highlights <str<strong>on</strong>g>the</str<strong>on</strong>g> fact that acute c<strong>on</strong>centrati<strong>on</strong>s of<br />

aqueous extracts of J. tanjorensis have moderate<br />

deleterious c<strong>on</strong>sequences <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> haema<str<strong>on</strong>g>to</str<strong>on</strong>g>logical<br />

parameters of C. gariepinus.<br />

Advances in studies <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> res<str<strong>on</strong>g>to</str<strong>on</strong>g>rati<strong>on</strong> of a<br />

salt lake ecosystem by introducing Artemia<br />

in Tibet<br />

Shasha LIU, Qinxian JIA, Xifang LIU, Xinsheng<br />

NIU <str<strong>on</strong>g>and</str<strong>on</strong>g> Mianping ZHENG<br />

MLR Key Labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry of Saline Lake Resources <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

Envir<strong>on</strong>ments, Institute of Mineral Resources, CAGS, Beijing<br />

100037, China. Email: liushashajida@163.com<br />

There are a lot of salt lakes in Tibet, some of which are<br />

enclosed by steep mountains <str<strong>on</strong>g>and</str<strong>on</strong>g> thus develop in<str<strong>on</strong>g>to</str<strong>on</strong>g><br />

relatively restricted envir<strong>on</strong>ments. It is difficult for <str<strong>on</strong>g>the</str<strong>on</strong>g>m<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> exchange with outside drainage basins, which results in<br />

low biodiversity <str<strong>on</strong>g>and</str<strong>on</strong>g> a single lower trophic level. In order<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> promote <str<strong>on</strong>g>the</str<strong>on</strong>g> salt lake ecosystem biodiversity <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

protect <str<strong>on</strong>g>the</str<strong>on</strong>g> fragile ecological envir<strong>on</strong>ment suffered from<br />

stress of ec<strong>on</strong>omic growth in nor<str<strong>on</strong>g>the</str<strong>on</strong>g>rn Tibet, <str<strong>on</strong>g>the</str<strong>on</strong>g> studies<br />

of introduced organisms with higher trophic levels <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

certain ec<strong>on</strong>omic value was implemented. Dangxi<strong>on</strong>g Co<br />

salt lake, our preferred experimental site, is surrounded<br />

<strong>on</strong> 3 sides by steep mountains <str<strong>on</strong>g>and</str<strong>on</strong>g> its eco‐isl<str<strong>on</strong>g>and</str<strong>on</strong>g> effect is<br />

obvious. After <str<strong>on</strong>g>the</str<strong>on</strong>g> argument of ecological risks <str<strong>on</strong>g>and</str<strong>on</strong>g><br />

simulated experiment of ecological effects during 2003<br />

<str<strong>on</strong>g>and</str<strong>on</strong>g> 2004. In 2004, we introduced 850 g Artemia cysts<br />

in<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> lake <str<strong>on</strong>g>and</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>y <str<strong>on</strong>g>the</str<strong>on</strong>g>n formed relatively stable<br />

natural populati<strong>on</strong>s, which perfected <str<strong>on</strong>g>the</str<strong>on</strong>g> local ecosystem<br />

through bringing in numbers of waterfowl in 2009. At<br />

present we report <str<strong>on</strong>g>the</str<strong>on</strong>g> research progress up <str<strong>on</strong>g>to</str<strong>on</strong>g> 2011.<br />

The data of 25 sites was collected during <str<strong>on</strong>g>the</str<strong>on</strong>g> period<br />

ofOc<str<strong>on</strong>g>to</str<strong>on</strong>g>ber–November. Based <strong>on</strong> data analysis of different<br />

water layers, <str<strong>on</strong>g>the</str<strong>on</strong>g> Artemia polypides <str<strong>on</strong>g>and</str<strong>on</strong>g> cysts distributed<br />

mainly in <str<strong>on</strong>g>the</str<strong>on</strong>g> surface water layer of 0.0–0.2m, <str<strong>on</strong>g>and</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

average biomass was 1.146×10 3 ind•m ‐2 <str<strong>on</strong>g>and</str<strong>on</strong>g> 2.590×10 4<br />

ind•m ‐2 respectively, <str<strong>on</strong>g>the</str<strong>on</strong>g> ratio were 24.62% <str<strong>on</strong>g>and</str<strong>on</strong>g> 30.81%<br />

respectively in <str<strong>on</strong>g>the</str<strong>on</strong>g> water column biomass. Biomass<br />

decreased c<strong>on</strong>tinuously with increasing depth in<br />

middle‐layer water <str<strong>on</strong>g>and</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> average biomass of polypides<br />

(2.0–8.0m) <str<strong>on</strong>g>and</str<strong>on</strong>g> cysts (2.0–10.0m) was <strong>on</strong>ly 2.969×10 2<br />

ind•m ‐2 <str<strong>on</strong>g>and</str<strong>on</strong>g> 5.958×10 3 ind•m ‐2 respectively, which<br />

accounted for 25.52% <str<strong>on</strong>g>and</str<strong>on</strong>g> 28.36% respectively in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

water column biomass. However, a slightly increasing<br />

trend in <str<strong>on</strong>g>the</str<strong>on</strong>g> 10–12m deep‐layer water was shown. Few<br />

polypide <str<strong>on</strong>g>and</str<strong>on</strong>g> cyst distributed in <str<strong>on</strong>g>the</str<strong>on</strong>g> water layer <strong>on</strong><br />

sediment over 12m in depth. The horiz<strong>on</strong>tal distributi<strong>on</strong><br />

characteristics of polypides <str<strong>on</strong>g>and</str<strong>on</strong>g> cysts in Dangxi<strong>on</strong>g Co<br />

92

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