Welcome to the 31st IUBS General Assembly and Conference on ...

Jing‐Shiun JAN, 1 Che‐Jen HSIAO, 2,3 Ya‐Ting KO, 1
Tzong‐Huei LEE 4 <strong>and</strong> George HSIAO 1
Shirleen YU
1Graduate Institute of Medical Sciences <strong>and</strong> Department of
Pharmacology, College of Medicine, Taipei Medical University,
Taipei, 2 School of Respira<strong>to</strong>ry Therapy, College of Medicine,
Taipei Medical University,Taipei, 3Division of Pulmonary
Medicine, Department of Internal Medicine, Taipei Medical
University Hospital, Taipei<strong>and</strong> 4Graduate Institute of
Pharmacognosy, Taipei Medical University, Taipei. Email:
geohsiao@tmu.edu.tw
There is much evidence indicating that activated human
monocytes/macrophages syn<strong>the</strong>size, <strong>and</strong> secrete several
pro‐inflamma<strong>to</strong>ry cy<strong>to</strong>kines <strong>and</strong> matrix
metalloproteinases (MMPs), <strong>and</strong> participate in <strong>the</strong>
inflamma<strong>to</strong>ry diseases. In this study, we investigated <strong>the</strong>
effects <strong>and</strong> mechanism of radicicol, as a heat shock
protein 90 (HSP90) inhibi<strong>to</strong>r, extracted from Nectria
balsamea #YMJ94052402, on human monocytic MMPs
<strong>and</strong> pro‐inflamma<strong>to</strong>ry cy<strong>to</strong>kine expression. Radicicol
(0.1‐5 μM) exhibited concentration‐dependent inhibition
of MMP‐9 enzymatic activation induced by tumor
necrosis fac<strong>to</strong>r‐α (TNF‐α), interleukin‐1β (IL‐1β) or
lipopolysaccharide (LPS) in human monocytic (THP‐1)
cells. Western blot analysis showed that radicicol
concentration‐dependently suppressed monocytic MMP‐9
protein expression. It was also found that radicicol
enhanced HSP70 expression in LPS‐activated monocytic.
Fur<strong>the</strong>rmore, we also found that radicicol could
significantly reduce <strong>the</strong> degradation of inhibi<strong>to</strong>r‐κB‐α
(IκB‐α) induced by LPS. In addition, we found that
radicicol showed reduction of <strong>to</strong>tal Akt expression in
LPS‐stimulated THP‐1 cells. On <strong>the</strong> o<strong>the</strong>r h<strong>and</strong>,
enzyme‐linked immunosorbent assay (ELISA) showed
that radicicol presented an inhibi<strong>to</strong>ry effect on ei<strong>the</strong>r
TNF‐α or IL‐6 production. However, radicicol exerted
with no significant effect on TNF‐α expression induced by
LPS in hyper<strong>to</strong>nic condition. In conclusion, we
demonstrate that radicicol attenuate MMP‐9 expression
<strong>and</strong> production of pro‐inflamma<strong>to</strong>ry cy<strong>to</strong>kines. Its main
mechanism might involve interference with <strong>the</strong> NF‐κB or
PI3K/Akt pathways. These results provide new
opportunities for <strong>the</strong> development of new
anti‐inflamma<strong>to</strong>ry strategies.
ThermoFisher Scientific
117
Thermo Fischer Scientific, Inc., Beijing, China. Email:
shirleen.yu@<strong>the</strong>rmofisher.com
Thermo Fisher Scientific
Inc. (NYSE: TMO) is <strong>the</strong>
world leader in serving
science. Our mission is <strong>to</strong>
enable our cus<strong>to</strong>mers <strong>to</strong> make <strong>the</strong> world healthier, cleaner
<strong>and</strong> safer. With revenues of $12 billion, we have
approximately 39,000 employees <strong>and</strong> serve cus<strong>to</strong>mers
within pharmaceutical <strong>and</strong> biotech companies, hospitals
<strong>and</strong> clinical diagnostic labs, universities, research
institutions <strong>and</strong> government agencies, as well as in
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Timing <strong>and</strong> synchrony of births in Père
David’s deer
Zhenyu ZHONG
Beijing Milu Ecological Research Center, Beijing Milupark, Nan
Haizi, Daxing, Beijing, China. Email: zhyzh@milupark.org.cn
Neonatal fawns of Père David’s deer (Elaphurusdavidianus)
in Nanhaizi Milupark, Beijing, China, were weighted <strong>and</strong>
marked, <strong>and</strong> timing <strong>and</strong> synchrony of births were
examined during 11 consecutive years (1998–2008).
Duration <strong>and</strong> timing of birth seasons varied among years.
Nei<strong>the</strong>r among‐year differences in average date of
fawning nor among‐year differences in <strong>the</strong> dispersion of
births occurred. Births were synchronized in all years;
80% of births <strong>to</strong>ok place in