In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

110 Chapter 8: SNA and refinement of titration protocol
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Figure 21: Diagrammatic representation of normal PBMC profile on cell size and granularity
(Courtesy of http://www.uni-greifswald.de/~immuteach/methods/facs/images/02facs.jpg ).
upward shift (cells becoming more granular) of a cluster of cells which could be indicative of
apoptotic PBMC (Ormerod, 1994) in the population kept at 37°C. Figure 21 is a diagrammatic
presentation of typical regions of PBMC subsets. It was further noted that the 37°C sample gave a
lower mean fluorescence: 134 compared to 331.7 CFSE log units of the sample stored at 4°C.
8.3.2. Exclusion of Con-A
Examination of smears from the two procedures revealed a clearer presentation of cells when
Con-A is excluded (Figure 22).
A
B
Figure 22: Cyto-centrifuged smears of Theileria parva infected lymphoblasts x400 (A)
stimulated with Con-A and (B) not stimulated. Infected cells are easily spotted in (B) unlike
in (A).