In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

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IN VITRO QUANTITATION OF THEILERIA PARVA SPOROZOITES FOR USE IN VACCINATION AND SPOROZOITE NEUTRALISATION ASSAYS Victor MBAO Proefschrift voorgedragen tot het behalen van de graad van Doctor in de Diergeneeskundige Wetenschappen, 2006 Promotoren: Prof. Dr. P. Dorny Dr. T. Marcotty Laboratorium voor Parasitologie Vakgroep Virologie, Parasitologie en Immunologie Faculteit Diergeneeskunde, Universiteit Gent
II Front cover photograph Copyright Institute of Tropical Medicine, Department of Animal Health
Page 1: Victor Mbao In vitro quantitation o
Page 6 and 7: V ACKNOWLEDGEMENTS This work was ma
Page 8 and 9: VII TABLE OF CONTENTS ACKNOWLEDGEME
Page 10: IX APPENDIX I .....................
Page 14 and 15: General Introduction 13 ___________
Page 16 and 17: General Introduction 15 ___________
Page 18: Chapter 1 - Quantitation of Theiler
Page 21 and 22: 20 Chapter 1: Quantitation of Theil
Page 44 and 45: Chapter 2: Objectives 43 __________
Page 46: Chapter 3 -Comparison of two Theile
Page 49 and 50: 48 Chapter 3: Theileria parva extra
Page 51 and 52: 50 Chapter 3: Theileria parva extra
Page 53 and 54:
52 Chapter 3: Theileria parva extra
Page 55 and 56:
54 Chapter 3: Theileria parva extra
Page 58 and 59:
Chapter 4: Suspension media and mul
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
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Chapter 5 - Snap freezing of Theile
Page 71 and 72:
70 Chapter 5: Snap freezing of Thei
Page 73 and 74:
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Page 78 and 79:
Chapter 6: Storage of Theileria par
Page 80 and 81:
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Page 88 and 89:
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Page 94 and 95:
Chapter 7: Lyophilisation of Theile
Page 96 and 97:
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Chapter 8: SNA and refinement of ti
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Chapter 9: General Discussion 119 _
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References 129 ____________________
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References 131 ____________________
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References 133 ____________________
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References 135 ____________________
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References 137 ____________________
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References 139 ____________________
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References 141 ____________________
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References 143 ____________________
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References 145 ____________________
Page 148:
APPENDICES
Page 151 and 152:
150 Appendices ____________________
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152 Appendices ____________________
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154 Appendices ____________________
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156 Appendices ____________________
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158 Appendices ____________________
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160 Appendices ____________________
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162 Appendices ____________________
Page 166:
SUMMARY
Page 169 and 170:
168 Summary _______________________
Page 171 and 172:
170 Summary _______________________
Page 174 and 175:
Samenvatting 173 __________________
Page 176 and 177:
Samenvatting 175 __________________
Page 178:
Curriculum Vitae
Page 181 and 182:
180 Carriculum Vitae ______________
Page 184 and 185:
List of Abbreviations 183 _________
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