In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

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Chapter 4: Suspension media and multiple freezing of Theileria parva stabilates 63 ______________________________________________________________________________________________ 1 Predicted proportion of positive wells 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 -6.5 -5.5 -4.5 -3.5 -2.5 -1.5 Dose (ln t.e.) Figure 8: Titration curves of RPMI (thin continuous line), MEM (thick broken line), FCS (thick continuous line) and PBS (thin broken line) based sporozoite stabilates. 1 0.9 Predicted proportion of positive wells 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 -6.5 -6 -5.5 -5 -4.5 -4 -3.5 -3 Dose(ln t.e.) Figure 9: Titration curves of stabilate that underwent a single refreeze/thaw cycle (broken line) and control stabilates (1.5 h storage on ice) (solid line).
64 Chapter 4: Suspension media and multiple freezing of Theileria parva stabilates ______________________________________________________________________________________________ A single re-freezing cycle resulted in a significantly lower infectivity (P=0.03) than the control kept on ice for 1.5 h (Fig. 9). The ED ratio was 0.68 (95 % CI: 0.48 – 0.95) and the estimated loss in infectivity was 32 %. The loss during multiple freeze cycles was also significant (P
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Victor Mbao In vitro quantitation o
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II Front cover photograph Copyright
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V ACKNOWLEDGEMENTS This work was ma
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VII TABLE OF CONTENTS ACKNOWLEDGEME
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IX APPENDIX I .....................
Page 14 and 15: General Introduction 13 ___________
Page 16 and 17: General Introduction 15 ___________
Page 18: Chapter 1 - Quantitation of Theiler
Page 21 and 22: 20 Chapter 1: Quantitation of Theil
Page 44 and 45: Chapter 2: Objectives 43 __________
Page 46: Chapter 3 -Comparison of two Theile
Page 49 and 50: 48 Chapter 3: Theileria parva extra
Page 58 and 59: Chapter 4: Suspension media and mul
Page 68: Chapter 5 - Snap freezing of Theile
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Page 78 and 79: Chapter 6: Storage of Theileria par
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Page 94 and 95: Chapter 7: Lyophilisation of Theile
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Page 104 and 105: Chapter 8: SNA and refinement of ti
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Chapter 8: SNA and refinement of ti
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Chapter 8: SNA and refinement of ti
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Chapter 9: General Discussion 119 _
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APPENDICES
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SUMMARY
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Curriculum Vitae
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180 Carriculum Vitae ______________
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List of Abbreviations 183 _________
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