In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

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List of Abbreviations 183 ______________________________________________________________________________________________ ANOVA Analysis of Variance ASVEZA Assistance to the Veterinary Services of Zambia BSA Bovine Serum Albumin CFSE Carboxyfluoroscein Succinimidyl Ester (Synonym CFDA-FE) CI Confidence Interval CL Confidence Limit Con-A Concanavalin A CPA Cryoprotectant Additive CTL Cytotoxic Lymphocyte DNA Deoxyribonucleic Acid ECF East Coast fever ED Effective Dose ELISA Enzyme-Linked ImmunoSorbent Assay EMA Ethidium Monoazide FAO Food and Agriculture Organisation FCS Fetal Calf Serum FSC Forward Scatter GLLAMM Generalised Linear Latent and Mixed Models HEPES 4-(2-Hydroxyethyl)-1-PiperazineEthanesulfonic acid I & T Infection and Treatment Method IFAT Immunofluorescent Antibody Test ITM Institute of Tropical Medicine L-15 Liebovitz medium ln t.e. Natural logarithm of the tick equivalent MEM Minimum Essential Medium MHC Major Histocompatibility Complex MSI Macro-schizont Index OIE International Office for Epizootics p67 67kDa circum-sporozoite immuno-dominant protein PBMC Peripheral Blood Mononuclear Cells PBS Phosphate Buffered Saline PCD Programmed Cell Death
184 List of Abbreviations ______________________________________________________________________________________________ PCR RPMI 1640 SCID SELISA SNA SSC t.e. Polymerase Chain Reaction Roswell Park Memorial Institute medium Severe Combined Immuno-deficiency Slide Enzyme-Linked Immunosorbent Assay Sporozoite Neutralization Assay Side Scatter Tick Equivalent
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Victor Mbao In vitro quantitation o
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II Front cover photograph Copyright
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V ACKNOWLEDGEMENTS This work was ma
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VII TABLE OF CONTENTS ACKNOWLEDGEME
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IX APPENDIX I .....................
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General Introduction 13 ___________
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General Introduction 15 ___________
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Chapter 1 - Quantitation of Theiler
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20 Chapter 1: Quantitation of Theil
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Chapter 2: Objectives 43 __________
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Chapter 3 -Comparison of two Theile
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48 Chapter 3: Theileria parva extra
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Chapter 4: Suspension media and mul
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Chapter 5 - Snap freezing of Theile
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70 Chapter 5: Snap freezing of Thei
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Chapter 6: Storage of Theileria par
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Chapter 7: Lyophilisation of Theile
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Chapter 8: SNA and refinement of ti
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Chapter 9: General Discussion 119 _
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References 129 ____________________
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Page 148: APPENDICES
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Page 166: SUMMARY
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Page 178: Curriculum Vitae
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In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

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