In vitro quantitation of Theileria parva sporozoites for use - TropMed ...
In vitro quantitation of Theileria parva sporozoites for use - TropMed ...
In vitro quantitation of Theileria parva sporozoites for use - TropMed ...
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112 Chapter 8: SNA and refinement <strong>of</strong> titration protocol<br />
______________________________________________________________________________________________<br />
Table 13. Comparison <strong>of</strong> PCR and smear reading <strong>for</strong> in <strong>vitro</strong> titrations.<br />
Smear PCR<br />
Total Cohen's kappa<br />
+ –<br />
Sucrose<br />
Trehalose<br />
Glycerol<br />
+<br />
+<br />
+<br />
14<br />
17<br />
9<br />
0<br />
5<br />
3<br />
14<br />
22<br />
12<br />
–<br />
–<br />
–<br />
0<br />
0<br />
2<br />
10<br />
2<br />
18<br />
10<br />
2<br />
20<br />
1<br />
0.42<br />
0.66<br />
Global<br />
+ 40 8 48<br />
– 2 30 32<br />
0.76<br />
Cohen's kappa<br />
0.8 almost perfect<br />
8.3.5. Sporozoite Neutralisation Assay<br />
No significant neutralisation relative to the control serum (FCS) was detected <strong>for</strong> all six test sera<br />
(P>0.05). The positive control serum (p67) was 100% inhibitory (P
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