In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

86 Chapter 6: Storage of Theileria parva stabilates at 4°C and -20°C
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1
0.9
Predicted proportion of protected animals
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0.0056 0.0058 0.006 0.0062 0.0064 0.0066 0.0068 0.007 0.0072 0.0074
ED 50 (tick equivalents)
Figure 15: Correlation between proportions of protected animals against in vitro ED 50.
1
storage at -20 o C
Proportion of positive wells
0.5
0
-8 -7 -6 -5 -4 -3 -2
Dose (ln t.e.)
.
0 1 2 4
Figure 16: Titration curves for Theileria parva sucrose stabilate stored at -20ºC for 1, 2 and 4
weeks.