In vitro quantitation of Theileria parva sporozoites for use - TropMed ...

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Appendices 155 _______________________________________________________________________________________________ APPENDIX III Titration curves for Theileria parva sporozoites suspended in four media: MEM, RPMI, PBS and FCS (as depicted in Figure 8 of chapter 4). MEM RPMI 1 0.5 Predicted proportion of positive wells 0 1 0.5 PBS FCS 0 -8.0 -6.0 -4.0 -2.0 -8.0 -6.0 -4.0 -2.0 Stabilate dose (In t.e.) ■-Predicted values, ○- Batch 2, X-Batch 1(a) and ∆-Batch 1(b)
156 Appendices ______________________________________________________________________________________________ APPENDIX IV Titration curves of multiply refrozen Theileria parva stabilates (Cycles 1 to 5) as depicted in Figure 10 of chapter 4. 1 1 2 0.5 0 1 Predicted proportion of positive wells 0.5 0 3 4 -6.2 -5.4 -4.6 -3.8 -3.0 1.0 5 0.5 0.0 -6.2 -5.4 -4.6 -3.8 -3.0 Stabilate dose (ln t.e.)
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Victor Mbao In vitro quantitation o
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II Front cover photograph Copyright
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V ACKNOWLEDGEMENTS This work was ma
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VII TABLE OF CONTENTS ACKNOWLEDGEME
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IX APPENDIX I .....................
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General Introduction 13 ___________
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General Introduction 15 ___________
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Chapter 1 - Quantitation of Theiler
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20 Chapter 1: Quantitation of Theil
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Chapter 2: Objectives 43 __________
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Chapter 3 -Comparison of two Theile
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48 Chapter 3: Theileria parva extra
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Chapter 4: Suspension media and mul
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Chapter 5 - Snap freezing of Theile
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70 Chapter 5: Snap freezing of Thei
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Chapter 6: Storage of Theileria par
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Chapter 7: Lyophilisation of Theile
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Chapter 8: SNA and refinement of ti
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Page 120 and 121: Chapter 9: General Discussion 119 _
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Page 130 and 131: References 129 ____________________
Page 148: APPENDICES
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Page 166: SUMMARY
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Page 178: Curriculum Vitae
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Page 184 and 185: List of Abbreviations 183 _________
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