(log ppb) HPRT VF (x 10

Metabolism Studies:
Liver in vitro metabolism systems
Model
Isolated perfused
liver
Liver slices
Hepatocytes
Liver cell lines
Subcellular fractions
Genetically
engineered cells
Liver in vitro metabolism systems
Characteristics
Ex vivo organ culture; complete liver enzymes and cofactors; bile flow
Closest to in vivo
Ex vivo tissue culture; complete liver enzymes and cofactors; cell-cell
interactions, cryopreservation limited - Close to in vivo,
Ex vivo cell culture; from livers or biopsies; complete liver enzymes
and cofactors; cryopreservation possible – Cell culture
In vitro cell culture; variable enzyme spectra; enzyme activities
limited; genotype instability; cryopreservation possible – Cell Culture
Protein suspension; ER-bound and cytosolic enzymes; lack of
cofactors; cryopreservation possible – enzyme preparation
In vitro cell culture or protein suspension; Enzymes produced in ER
of host cells (bacteria, yeast, mammalian cell lines or baculovirus);
with or without cofactors – arteficial system
Modified according to Guillouzo A (1998) Environmental Health Perspectives 106(2), 511-532. and Coecke S et al. (2006) ATLA 34, 49-84.