European Journal of Medical Research - Deutsche AIDS ...

70 EUROPEAN JOURNAL OF MEDICAL RESEARCH
June 27, 2007
Result: 41 (82%) out of 50 females were positive to Herpes
IgG out of which 28(68.3%) were positive to IgM. 20 out of
12(93.3%) were positive to Herpes IgG out of which
7(63.6%) were positive to IgM. 37(94.9%) out of 39 females
were positive to CMV IgG out of which 21 (56.8%) were positive
to IgM. 12 (93.9%) out 13 males were positive to CMV
IgG out of which 7 (58.3%) were positive to CMV IgM.
30(76.9%) out of 39 females were positive to Rubella IgG out
of which 9(26.5%) were positive to Rubella IgM. 11(91.7%)
out of 12 males were positive to Rubella IgG out of which
10(93.9%) were positive to IgM. 14(58.3%) out of 24 females
were positive to Varicella IgG out of which 5(20.8%) were
positive to Varicella IgM. 8(72.7%) out of 11 males were positive
to Varicella IgG out of 1(12.5%) was positive to Varicella
IgM. 25(65.8%) out of 38 females were positive to Mumps
IgG out of which 17(53.1%) were positive to the IgM.
12(93.3%) out of 13 males were positive to Mumps IgG out
of which 6(50%) were positive to the IgM. 27(67.5%) out of
40 females were positive to Toxoplasmosis IgG out of which
15(42.9%) were positive to the IgM. 12(93.3%) out of 13 females
were tested positive and 2(50%) out of 10 IgG positive
were tested positive to the IGM.
31(72.1%) out of 43 females were tested positive to
Chlamydia IgG out of which 22(61.1%) were positive to the
IgM. 8(57.4%) out of 14 males were positive to Chlamydia
IgG, 2(40%) out 5 IgG positive tested positive to the IgM.
None(0%) out of 38 females tested positive to Hepatitis B and
Hepatitis C. 2(16.7%) out of 12 males were positive to Hepatitis
and none was positive to Hepatitis C. 4(10.3%) out of
39 females were positive to Syphilis. 3(27.3%) out of 11
males were positive to Syphilis
Conclusion: Our study shows that HIV positive patients are
carriers of other antibodies and should be screened for them
before therapy.
C.2 (Vortrag)
GBV-C co-infection in HIV patients downregulates
CCR5 and CXCR4 surface expression on CD4 cells
Schwarze-Zander C. 1 , Neibecker M. 1 , Othman S. 1 ,
Schulz M.1, Voigt E. 1 , Vogel M. 1 , Wasmuth J.-C. 1 ,
Lüchters G. 2 , Rockstroh J.K. 1 , Spengler U. 1
1 Medizinische Klinik und Poliklinik I, Universität Bonn,
Bonn, Germany, 2 Universität Bonn, Bonn, Germany
Background: The flavivirus GB virus-C (GBV-C), thus far
not known to cause any disease, has been shown to be associated
with delayed progression of HIV disease. Recently, in
vitro studies demonstrated down-regulation of CCR5 as a potential
mechanism of GBV-C to modulate HIV disease progression.
We therefore studied surface expression of the two
major HIV co-receptors, CCR5 and CXCR4, on CD4+ and
CD8+ T-cells in 110 HIV patients stratified with respect to
their GBV-C status and immune function.
Methods: GBV-C infection was studied in 110 HIV patients
by RT-PCR. CCR5D32 mutation was analyzed by real time
PCR. FACS analysis was used to measure CCR5 and CXCR4
surface expression on CD4+ and CD8+ T-cells.
Results: GBV-C RNA replication was detected in 31%
(34/110) of patients. Fourteen patients were excluded from
the analysis because of reduced CCR5 surface expression due
to a heterozygous CCR5D32 mutation. In the remaining
HIV/GBV-C co-infected patients with CD4