Software application manual - VTP UP

7. Application Examples7.1 OverviewThis chapter provides examples of the use of the Coulochem III detector to controlelectrochemical (EC) cells with fixed (DC) or dynamic (pulsed) potential waveforms. Cellscan be used as reactors in series upstream of the MS ion source and/or as detectors in parallel(via flow split) with MS. Common applications are for on-line derivatization (e.g., to assistMS ionization, for mass tagging and protein cleavage) and to study the susceptibility ofcompounds to oxidation (or reduction), the nature of products and short-lived intermediates,and the associated reaction pathways. These applications are of interest for generating andcharacterizing products that often correspond to biological metabolites, degradants or reactivespecies of interest. The use of EC in parallel with MS provides complementary, orthogonal,detection with high sensitivity and selectivity for redox active species. Typical applicationsare DC detection of antioxidants and markers of oxidative stress and pulsed detection ofcarbohydrates.Each example tabulates the conditions for the liquid chromatography (LC) setup, CoulochemIII (EC) setup and mass spectrometer (MS) setup.Coulochem ® III Analyst Driver - Version 1.0 49User’s Manual