Software application manual - VTP UP

Chapter 7LC Conditions• Column: Shiseido C18 MG 3µm 4.6 mm ID x 75 mm• Mobile phase gradient:o A: 1% ACN, 20 mM NH 4 Acetateo B: 80% ACN, 20 mM NH 4 AcetateFlowTime %B (ml/min)0.00 20 110.00 100 112.00 100 112.01 20 115.00 20 1• Flow rate: 1 ml/min• Injection: 10 µl Tamoxifen, 20 µg/ml (200 ng)• After diode array, flow splitter (ESA P/N 70-6228) adjusted to allow 200 µl intothe MS.EC Conditions• ESA Model 5021A Conditioning (Reactor) Cell• Ground Decoupling Kit (ESA P/N 70-5971) connected between EC cell and MS.• Cell potential: 500 mV, repeat at 1000 mV• Filter time constant: 0.5 sec• Full scale gain range: 10 µA• Signal output voltage: +1V• Baseline offset: 0 %• Pre-equilibration: 2 min, autozero prior to runMS Setup• Electrospray: positive ion mode• Enhanced MS Scan 100 to 500 m/z52 Coulochem ® III Analyst Driver – Version 1.0User’s Manual