Software application manual - VTP UP

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Chapter 7LC Conditions• Column: Shiseido C18 MG 3µm 4.6 mm ID x 75 mm• Mobile phase gradient:o A: 1% ACN, 20 mM NH 4 Acetateo B: 80% ACN, 20 mM NH 4 AcetateFlowTime %B (ml/min)0.00 20 110.00 100 112.00 100 112.01 20 115.00 20 1• Flow rate: 1 ml/min• Injection: 10 µl Tamoxifen, 20 µg/ml (200 ng)• After diode array, flow splitter (ESA P/N 70-6228) adjusted to allow 200 µl intothe MS.EC Conditions• ESA Model 5021A Conditioning (Reactor) Cell• Ground Decoupling Kit (ESA P/N 70-5971) connected between EC cell and MS.• Cell potential: 500 mV, repeat at 1000 mV• Filter time constant: 0.5 sec• Full scale gain range: 10 µA• Signal output voltage: +1V• Baseline offset: 0 %• Pre-equilibration: 2 min, autozero prior to runMS Setup• Electrospray: positive ion mode• Enhanced MS Scan 100 to 500 m/z52 Coulochem ® III Analyst Driver – Version 1.0User’s Manual
Application ExamplesResultsFigure 7.2 shows results for 200 ng Tamoxifen with the EC cell at a potential of 500 mV.Two forms of Tamoxifen are primarily observed, at m/z 358.8 (A) and 372.8 (B).4e+63e+62e+6cps1e+6EC Potential 500mVTICAB00 2 4 6 8 10 12 14Time (min)1201008060AXICm/z 358.8ONHCH340200100 200 300 400m/zCH3120100 B XIC m/z 372.880OCH3NCH360CH340200100 200 300 400m/zFigure 7.2. Tamoxifen at a Cell Potential of 500 mVCoulochem ® III Analyst Driver – Version 1.0 53User’s Manual
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Coulochem ® III Analyst ® DriverV
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WarrantyA. ESA Biosciences, Inc. (
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License AgreementESA BIOSCIENCES, I
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License AgreementBY CUSTOM OR TRADE
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Table of Contents1. Introduction...
Page 11 and 12: 1. Introduction1.1 OverviewThis man
Page 13 and 14: Introductioncursor (over the Auto Z
Page 15 and 16: 2. System Requirements and Installa
Page 17 and 18: System Requirements and Installatio
Page 19 and 20: System Requirements and Installatio
Page 21: System Requirements and Installatio
Page 24 and 25: Chapter 3Figure 3.1. Status Bar Sho
Page 26 and 27: Chapter 33.4 Configuring the Couloc
Page 29 and 30: 4. Creating and Modifying Acquisiti
Page 31 and 32: Creating and Modifying Acquisition
Page 49 and 50: 5. Basic Coulochem III Driver Funct
Page 51: Basic Coulochem III Driver Function
Page 54 and 55: Chapter 6Instructions for building
Page 56 and 57: Chapter 6Figure 6.4. Acquisition St
Page 58 and 59: Chapter 6To view the method propert
Page 60 and 61: Chapter 77.2 Assisted Ionization -
Page 64 and 65: Chapter 7Oxidation at 1000 mV produ
Page 66 and 67: Chapter 7ResultsFigure 7.4 shows th
Page 68 and 69: Appendix AC:\Program Files\ESA Bios
Page 71 and 72: Appendix B: Parameter Ranges and De
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