FINAL PROGR AM - American Society of Gene & Cell Therapy

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AMERICAN SOCIETY OF GENE THERAPY • Final Program 75 ABSTRACTS FRIDAY, JUNE 3 477 Development of SIV Based Vectors with Efficient Delivery for Vector Dynamic Studies in the Simian System Christopher Chen, Jiamo Lu, Tatiana Slepushkina, Andrew Worden, Vladimir Slepushkin, Laurent M. Humeau, Gwendolyn K. Binder, Xiaobin Lu 478 A HIV-1 Based Cross-Packaging System for FIV Vectors Adam S. Cockrell, Hong Ma, Thomas McCown, Kim D. Kluckman, Randy J. Thresher, Tal Kafri 479 Targeted Entry of Retroviral Vectors Depends on Characteristics of the Target Receptor and the Chimeric Envelope Protein Lorraine M. Albritton, Fang Li, Pankaj Kumar, Langzhu Tan 480 Examination of the Relationship between the Level of Gene Transfer and the Number of Envelope Proteins That Are Incorporated Per Retrovirus Particle When High Doses of Purified Virus Stocks Are Used to Transduce Cells Natalia Landázuri, Joseph M. Le Doux 481 Dynamics of Transduction of Human Cells by EIAV Vectors – The Importance of Cell Entry Daniel C. Farley, Fraser J. Wilkes, James E. Miskin, Susan M. Kingsman, Kyriacos A. Mitrophanous 482 Neuronal Gene Transfer with HIV-1-Based Lentiviral Vectors Pseudotyped with Lyssavirus Glycoproteins Qingshan Teng, Mary E Garrity-Moses, Thais Federici, Jun Yang, Robert Kutner, Noel Tordo, Jakob Reiser, Nicholas M. Boulis 483 Targeted Receptor Trafficking Affects the Efficiency of Retrovirus Transduction Delfi Krishna, Julia Raykin, Joseph Le Doux 484 Ultrasound-Mediated Microbubble Destruction and Retroviral Gene Delivery Sarah L. Taylor, Ahad Rahim, Nigel Bush, Jeff Bamber, Colin Porter 485 Lentivirus-Mediated Gene Transfer to Cultured Fetal Sheep Airway Epithelium and Lung Tissue Explants Ze-Yan Yu, Karen McKay, Peter Van Asperen, Maolin Zheng, Jane Fleming, Samantha Ginn, Eugenie Lumbers, Peter Rowe, Ian Alexander 486 Optimized Conditions for Retroviral Gene Transfer by RetroNectin ® Bound Virus Infection Method Hideto Chono, Hiromi Okuyama, Yasushi Katayama, Nobuto Koyama, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato 487 Enhancement of Adeno- and Lentivirus Mediated Gene Transfer in Human Tumor Cell Lines by Cell Permeable Peptides and Polycations Saara Lehmusvaara, Outi Meriläinen, Tanja Hakkarainen, Jarmo Wahlfors 488 Engineering Enhanced Retroviral Vectors through the Insertion of a His Tag into VSV-G 6 Julie H. Yu, David V. Schaffer 489 Further Studies of the Most Efficient Vector Systems for Gene Transduction into Human Dendritic Cells Line (im-NMD) Eigo Sato, Masanari Kato, Yuko Hara, Hua Yan, Tohko Miyagi, Xiao-Kang Li, Wataru Sugiura, Naoki Yamamoto, Kenichi Teramoto, Shigeki Arii Hiromitsu Kimura 490 Lentiviral-Mediated Gene Transfer to Cells of the Vasculature Ciara A. O’Shea, Robert J. Nelson, Padraig Strappe, Timothy O’Brien 491 Development of Fast and Efficient Methods for the Purification and Concentration of Lentiviral Vectors Rachel M. Koldej, Donald S. Anson 492 Harvest Timing and Media Composition Effects on Lentiviral Vector Production Christopher Ballas, Terry Johnson, Kenneth Cornetta 493 Concentration and Purification of Lentivirus Pseudotypes Bearing Alternative Envelope Glycoproteins Robert H. Kutner, Jakob Reiser 494 Assessing Lentiviral Vector Quality Using Transmission Electron Microscopy Lakshmi Sastry, Carole Miller, Terry Johnson, Aparna Jasti, Vince Gattone, Kenneth Cornetta 495 Upscaling of Retroviral Vector Production under GMP Guidelines to Generate High Titer Preparations for Clinical Use Sandra Jung, Peter Leyendecker, Frank Kogelberg, Christian Wolf, Andrea Schilz, Klaus Kuehlcke, Sonja Naundorf
76 8th Annual Meeting • June 1 - 5, 2005 • St. Louis ABSTRACTS FRIDAY, JUNE 3 496 Stability of Oncolytic Measles Viruses during Storage and Clinical Preparation Troy R. Wegman, Kirsten K. Langfield, Henry J. Walker, Guy E. Griesmann, Julie A. Sauer, Sharon A. Stephan, Mark J. Federspiel AAV Vectors: Vector Biology 497 Sex and Estrous Cycle Stage Influence the Efficiency of AAV-Mediated Gene Transfer in the Rodent Brain James C. Dodge, Jennifer Clarke, Marco A. Passini, Anthony Song, Catherine R. O’Riordan, Seng H. Cheng, Gregory R. Stewart 498 Adeno-Associated Virus Type-1 Vectors Efficiently Transduce Human Polarized Airway Epithelia without Polarity Bias Ziying Yan, Xiaoming Liu, Chi-Man Lei, Meihui Luo, John F. Engelhardt 499 Species-Specific Differences in the Polarity of rAAV5 and rAAV2 Transduction of Mouse and Human Airway Epithelia Xiaoming Liu, Ziying Yan, Meihui Luo, John F. Engelhardt 500 Biology of Novel AAV Serotype Vectors in the Liver Directed Gene Transfer to Nonhuman Primates Guangping Gao, You Lu, Roberto Calcedo, Rebecca Grant, Julio Sanmiguel, Lili Wang, Julie Johnston, Joanita Figueredo, Martin Lock, James M. Wilson 501 AAV6 Pseudotyped Double-Stranded-AAV Vectors Allow Adenoviral-Like Transduction and Brute-Force Integration in the Growing Mouse Ognjen Petras, Timothy J. Davern, Marcus O. Muench, Xiaodong Han 502 GFP-Tagged Adeno-Associated Viral (AAV) Particles Allow the Study of Cytosolic and Nuclear Trafficking Kerstin Lux, Stefanie Schlemminger, Luca Perabo, Daniela Goldnau, Jan Endell, Michael Hallek, Hildegard Buening 503 Mutational Strategy To Enhance or Rescue Functionality of Existing AAV Vectors Luk H. Vandenberghe, Michael H. Yang, Julie Johnston, Guangping Gao, James M. Wilson 504 In Vivo Characterization of AAV Serotype 9 Vectors in Mice Katsuya Inagaki, Theresa A. Storm, Sally Fuess, Mark A. Kay, Hiroyuki Nakai 505 Analysis of AAV Genome Conversion and Stabilization Bernd Hauck, Jing Xie, Weidong Xiao 506 Transductional and Genome Biodistribution of Novel AAV Serotype Vectors after Systemic Delivery and Their Liver Specific Dose Response Profiles in Mice You Lu, Guangping Gao, Julio Sanmiguel, Roberto Calcedo, Peter Bell, Nelson Wivel, James M. Wilson 507 Modeling Adeno-Associated Virus 2 Capsid for the Identification of Targeting Ligands for Enhanced Gene Transfer to Polarized Human Airway Epithelial Cells April F. White, Marina Mazur, Eric J. Sorscher, Selvarangan Ponnazhagan 508 Impact of Sex on Serum að1-Antitrypsin Levels Following Intrapleural and Intravenous Administration of AAVrh.10 Vectors to Mice Bishnu P. De, Adriana Heguy, Neil R. Hackett, Guangping Gao, James M. Wilson, Ronald G. Crystal 509 Endogenous Estradiol Augments RGD Modified AAV2- Mediated Gene Transfer in Human Ovarian Epithelial Cancer Wenfang Shi, Jeffery Bartlett 510 Neonatal Gene Transfer with Non-Human Primate AAV Vector Serotypes Results in Widespread Transduction and Gene Activity in the Mouse Brain Brian A. Karolewski, Hennessy Howell, John H. Wolfe 511 Effect of Hepatocyte Division on Molecular Fate of rAAV DNA Young-Kook Choi, Yuanqing Lu, Sihong Song 512 The Cellular TATA-Binding Protein Is Required for Replication of a Minimal Adeno-Associated Virus Type 2 (AAV-2) p5 Promoter Element Achille François, Mickael Guilbaud, Rafi Awedikian, Gilliane Chadeuf, Philippe Moullier, Anna Salvetti 513 Novel Characteristics of Heparin Binding by Adeno- Associated Virus Serotype 6 James M. Allen, Eric E. Finn, Jeffrey S. Chamberlain
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156 NOTES 8th Annual Meeting • Ju
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FINAL PROGR AM - American Society of Gene & Cell Therapy

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