Haematologica 2004;89: supplement no. 8 - Supplements ...

XVIII Congress of the Italian Society for Hemostasis and Thrombosis Research, Rome, Sept. 30-Oct. 3, 200495increased risk of being in the highest (> 19 mm) aorticdiameter group (OR= 4.0, 95% CI 1.3-11.7,p=0.02). High levels of CRP (top tertile vs lowest tertile)are associated, but not significantly, with anincreased risk of having high aortic diameter (OR=1.3; 95% CI 0.8-2.0 for aortic diameter >19 mm).These results strengthen the concept of a possiblelink between inflammation and aortic remodelling.PO-002EXPRESSION PROFILE OF DIFFERENT COAGULATION MARKERSIN HUMAN PLACENTAS FROM UNEVENTFUL PREGNANCIESChinni E, Colaizzo D, Bossone A, Vecchione G,Cocomazzi N, Martinelli P, Pecoraro M,Sciannamè N, Margaglione M, Grandone EIRCCS Casa Sollievo della Sofferenza, Ostetricia eGinecologia, Università Federico II Napoli, GeneticaMedica, Università degli studi di Foggia, ItalyPlacental throphoblasts express and produce coagulationcomponents, partecipating not only in hemostatsis,but also in the placental vascular developmentand differentiation. The expression of tissue factor(TF), membrane phosphatidylserine and fibrin renderthe throphoblasts pro-coagulant, thus compromisingthe risk of bleeding while exposing the placenta topro-thrombotic risks. Local inhibitory mechanisms actvia TFPI-1 and TFPI-2, thrombomodulin, annexi nV.Pregnancy complications have been associated withabnormalities in the functions of these inhibitors.Moreover, different isoforms of the thromboxane A2receptor seem to be differently expressed in mice withfetal growth restricted fetuses. The aim of our studywas to investigate whether different areas of placentaexpress different amounts of the same marker inorder to obtain information about homogeneity ofexpression. Here we present data obtained from 4 placentasof women with successful pregnancy outcome.The placentas were dissected starting from the inserctionof umbilical cord towards the peripheral zone.From each section RNA was extracted by means InvitrogenTrizol‚Reagent. Then cDNA was obtained bymeans RT-PCR (Promega Reverse trascription system),and the expression of TF, TFPI, TFPI-2, PAI-2, andthromboxane receptor α-isoform and β-isoform wasevaluated in different areas of placenta by means ofAPPLERA ABI PRISM 7700. Data were expressed aspercent of GAPDH for each sample analyzed. Data areshown in the Table. No significant difference (p>0.05)between central and peripheral areas of placentas wasobserved for all markers analysed. These preliminarydata will be useful for comparing the expression ofthese markers in normal placentas with that of placentasfrom complicated pregnancies.TF% TFPI% TFPI-2% PAI-2 TXA2R a TXA2R bMean SD Mean SD Mean SD Mean SD Mean SD Mean SDN 105 8,2 112,5 5,8 135 15,1 92 5,8 111 3,7 75 3,1A 105,3 25,6 114 2,6 136 4,7 93 1,8 86,4 4,4 76 3,6B 105,6 4,2 112,5 2,9 134 7,6 94,5 3,1 86,3 4 79 2,6Legenda: N: zone corresponding to the inserction of umbilical cord; A: zone next to N, B: marginalzone of placenta.PO-003PPAR-γ EXPRESSION AND MODULATION IN HUMANSTIMULATED FIBROBLASTSEvangelisti L, Giusti B, Bandinelli B, Attanasio M,Lapini I, Lucarini L, Cesari F, Filoni C, Ferrini S,Abbate R, Gensini GF, Pepe GDipartimento di Area Critica Medico Chirurgica,Università degli Studi di Firenze, ItalyPeroxisome proliferator-activated receptors-γ(PPAR-γ) is a nuclear transcription factor that isinvolved in inflammatory process by modulating theproduction of pro-inflammatory cytokines. Severalstudies have demonstrated that PPAR-gamma isexpressed by different human cells (adipose, endothelialand smooth muscle cells) and displays antiinflammatoryproperties. Recently, in rat sinovialfibroblasts, the expression of PPAR-γ and its LPSinducedreduction were demonstrated at both mRNAand protein levels. Data about PPAR-γ expression byhuman fibroblasts are not available. Aim of this studywas to investigate whether PPAR-γ is expressed byhuman skin and adventitial fibroblasts, and to evaluatethe modulation of PPAR-γ expression by differentinflammatory stimuli. For this purpose, we grewdermal fibroblasts from three healthy subjects andfibroblasts from the adventitia of three patientsaffected by abdominal aortic aneurysm. Fibroblastsfrom skin biopsy of controls and from adventitia ofpatients were cultured and incubated with differentinflammatory stimuli (LPS 10 µg/mL; TNF α 50 ng/mL+ IFN-γ 300 U/mL) and incubation times (0h, 6h,12h, 24h). PPAR-γ mRNA from fibroblasts wasassayed by a semi-quantitative RT-PCR method. TheRT-PCR products were quantitated after gel electrophoresisby using the GAPDH mRNA as internalcontrol. Time-course experiments with LPS and TNFα/IFN-γstimuli carried out on adventitial fibroblastsshowed an increase of PPAR-gamma expression at 6,12 and 24 hours respectively; also stimulated dermalfibroblasts displayed an increased PPAR-γ expression,but of minor intensity than adventitial fibroblasts.At the best of our knowledge, this is the firsthaematologica vol. 89(suppl. n. 8):september 2004