Haematologica 2004;89: supplement no. 8 - Supplements ...

XVIII Congress of the Italian Society for Hemostasis and Thrombosis Research, Rome, Sept. 30-Oct. 3, 2004155domized to undergo OPCAB (n=17) or CABG (n=18).Blood samples were collected before the interventionand up to one month after surgery. Prothrombinfragment F1.2 (F1.2), thrombin-antithrombin complex(TAT) and D-dimer (XDP) increased after surgeryand were persistently higher than preoperative valuesup to 30 postoperative days both in OPCAB andCABG; higher levels of these variables were detectedin CABG with respect to OPCAB only at the timepoint after termination of CPB (F1.2 and TAT) or fromCPB end up to 8 postoperative days (XDP). Fibrinogenlevels decreased after surgery, then increased inparallel in both groups up to 8 days after surgery. vonWillebrand factor (vWF) increased postoperatively inboth groups and returned to baseline 30 days aftersurgery, being higher in CABG from CPB end up to 8postoperative days; soluble Vascular Cell AdhesionMolecule-1 (sVCAM) increased significantly overbaseline in both groups 30 days after surgery with nodifference between groups. In conclusion OPCABpatients show a protection against activation ofcoagulation and fibrinolysis and of endothelium onlyduring the intraoperative period, followed by thedevelopment of a prothrombotic pattern comparableto that of CABG patients lasting at least up to 30days after surgery.PostersHEREDITARY THROMBOPHILIAPO-121FACTOR V LEIDEN AND G20210A PROTHROMBIN MUTATIONSIN LATE PREGNANCY LOSSSantoro R, Iannaccaro P, Sottilotta G, Papaleo GCentro Emofilia, Centro di Riferimento Regionaleper la Patologia Emorragica e Trombotica Ereditaria,Azienda Ospedaliera “Pugliese-Ciaccio”, CatanzaroIntroduction: The role of inherited trombophilias inthe occurrence of unexplained recurrent spontaneousabortions, fetal loss, abruptio placentae, intrauterinegrowth restriction (IUGR) and pre-eclampsia has beenstudied and established. Although fetal loss in the firsttrimester is a common complication of pregnancy andhas many possible causes, fetal loss in the second andthird trimesters is often associated with placentalinsufficiency. In the recent years a growing number ofreports have suggested that prothrombotic moleculardefects are associated with vascular pathologies,resulting in poor gestational outcome. The aim of thisstudy was to investigate the prevalence of factor VLeiden (FVL) and factor II prothrombin mutation (PTM)in women with at least one previous intrauterine fetaldeath, comparated with the prevalence of the samemutations in our control group. Methods: We have retrospectivelyevaluated 27 women with a history ofunexplained late pregnancy loss (fetal death at 20weeks or more of gestation), with a median age of 31years, and we have comparated the prevalence of theFVL mutation and of the FIIG20210A mutation in thesewomen and in a control group of 115 healthy womenwithout history of pregnancy loss. Markers for thethrombophilias were assessed by PCR analysis. Results:The FVL mutation and the FII G20210A mutation wereshown in 6/27 (22,2%) and in 5/27 (18,5%) womenwith history of late pregnancy loss, respectively, incomparison with a prevalence of 2,6% of both FVL andFII G20210A mutations in the control group. The differencewas statistically significant (p=0,0001 for theFVL and p=0,001 for the FII G20210A ). Conclusions: Inour experience the prevalence of FVL and the FIIG20210A mutations was significantly higher in womenwith unexplained late pregnancy loss, suggesting thatthe screening for these mutations might be indicatedin this setting.haematologica vol. 89(suppl. n. 8):september 2004