Haematologica 2004;89: supplement no. 8 - Supplements ...

74Oral Communicationsmorphism, but not in those with folic acid ≥5 nmol/L.Among all the possible combinations of the 3 analyzedpolymorphisms, the more frequent combinedgenotypes resulted 677CT/1298AC/2756AA (28.6%),677TT/1298AA/2756AA (19.6%) and 677CT/1298AA/2756AA (16.7%). Homocysteine levels were significantly(p0.05). Atthe multivariate logistic regression analysis adjustedfor age, sex, creatinine plasma levels, folate, B6and B12 plasma levels, folate and B6 intakes andMTHFR polymorphism, eNOS gene polymorphismswere not significantly associated with an increasedrisk of having high levels of homocysteine. Nevertheless,as far as subjects with lowest serum folatetertile are considered, only eNOS -786C variant influencedthe risk of being in the highest homocysteinetertiles (2 nd tertile: OR 6.4, p=0.02; 3rd tertile: OR8.3, p=0.01). Our results document a possible associationbetween eNOS T-786C polymorphism andhomocysteine plasma levels in healthy subjects withlow serum folate levels.CO-104A PROINFLAMMATORY STATE INDICATED BY HIGH IL-6LEVELS IS RELATED TO HYPERHOMOCYSTEINEMIAGori AM, Fedi S, Corsi AM,* Rogolino A,^Alessandrello Liotta A,^ Gazzini A, Lenti M, Poggi F,Rossi L, Ferrucci L,** Abbate R, Gensini GFDepartment of Medical and Surgical Critical Area,University of Florence; *Laboratory of ClinicalEpidemiology, Geriatric Department, National Instituteof Research and Care on Aging (INRCA), Florence;^Dipartimento Cardiologico e dei Vasi, AziendaOspedaliero-Universitaria Careggi, Florence,Italy; **Longitudinal Studies Section, GerontologyResearch Center, National Institute on Aging,National Institutes of Health, Baltimore, USAElevated levels of homocysteine may reflectincreasing age, male sex, renal failure, vitamin deficiencies(low folate, vitamin B12 or vitamin B6), orinherited abnormalities of enzymes involved in themethionine metabolism. Aim of this study was todetermine in a large population based samplewhether hyperhomocysteinemia is associated withhigh circulating levels of inflammatory markers.Westudied 586 men and 734 women randomly selectedfrom the people living in two sites in the surroundingof Florence, Italy. In the InCHIANTI populationhomocysteine, folic acid, vitamin B6 and B12levels were 13.7 (5.4-95.9) µmol/L, 6.3 (0.7-45.3)nmol/L, 25.3 (0.4-689.2) nmol/L and 285 (25.2-1488.1) pmol/L respectively. Dietary vitamin B6 andfolic acid intakes were strongly (p