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Haematologica 2004;89: supplement no. 8 - Supplements ...

Haematologica 2004;89: supplement no. 8 - Supplements ...

Haematologica 2004;89: supplement no. 8 - Supplements ...

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XVIII Congress of the Italian Society for Hemostasis and Thrombosis Research, Rome, Sept. 30-Oct. 3, <strong>2004</strong>1173) pyruvate+NADH (arrow) lactate+NADThe chromatographic procedure included reductio<strong>no</strong>f disulfide bonds by NaBH4, use of cysteamineas internal standard, derivatization with ammonium-7-fluorobenzo-2-oxa-diazole-4-sulphonate(SBD-F), and fluorescence detection after separationby reversed-phase HPLC. Medians and ranges of tHcyplasma levels (n=96) observed were: 16.1 (3.9-61.8)and 17.5 (4.9-58.3) µmol/L for HPLC and enzymickit, respectively. Linear regression analysis yielded:enzymic assay = 1.044 (± 0.546) + 1.011(± 0.024)HPLC (±SE), r = 0.975, Sx/y = 2.85 (p

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