XVIII Congress of the Italian Society for Hemostasis and Thrombosis Research, Rome, Sept. 30-Oct. 3, <strong>2004</strong>95increased risk of being in the highest (> 19 mm) aorticdiameter group (OR= 4.0, 95% CI 1.3-11.7,p=0.02). High levels of CRP (top tertile vs lowest tertile)are associated, but <strong>no</strong>t significantly, with anincreased risk of having high aortic diameter (OR=1.3; 95% CI 0.8-2.0 for aortic diameter >19 mm).These results strengthen the concept of a possiblelink between inflammation and aortic remodelling.PO-002EXPRESSION PROFILE OF DIFFERENT COAGULATION MARKERSIN HUMAN PLACENTAS FROM UNEVENTFUL PREGNANCIESChinni E, Colaizzo D, Bossone A, Vecchione G,Cocomazzi N, Martinelli P, Pecoraro M,Sciannamè N, Margaglione M, Grandone EIRCCS Casa Sollievo della Sofferenza, Ostetricia eGinecologia, Università Federico II Napoli, GeneticaMedica, Università degli studi di Foggia, ItalyPlacental throphoblasts express and produce coagulationcomponents, partecipating <strong>no</strong>t only in hemostatsis,but also in the placental vascular developmentand differentiation. The expression of tissue factor(TF), membrane phosphatidylserine and fibrin renderthe throphoblasts pro-coagulant, thus compromisingthe risk of bleeding while exposing the placenta topro-thrombotic risks. Local inhibitory mechanisms actvia TFPI-1 and TFPI-2, thrombomodulin, annexi nV.Pregnancy complications have been associated withab<strong>no</strong>rmalities in the functions of these inhibitors.Moreover, different isoforms of the thromboxane A2receptor seem to be differently expressed in mice withfetal growth restricted fetuses. The aim of our studywas to investigate whether different areas of placentaexpress different amounts of the same marker i<strong>no</strong>rder to obtain information about homogeneity ofexpression. Here we present data obtained from 4 placentasof women with successful pregnancy outcome.The placentas were dissected starting from the inserctio<strong>no</strong>f umbilical cord towards the peripheral zone.From each section RNA was extracted by means InvitrogenTrizol‚Reagent. Then cDNA was obtained bymeans RT-PCR (Promega Reverse trascription system),and the expression of TF, TFPI, TFPI-2, PAI-2, andthromboxane receptor α-isoform and β-isoform wasevaluated in different areas of placenta by means ofAPPLERA ABI PRISM 7700. Data were expressed aspercent of GAPDH for each sample analyzed. Data areshown in the Table. No significant difference (p>0.05)between central and peripheral areas of placentas wasobserved for all markers analysed. These preliminarydata will be useful for comparing the expression ofthese markers in <strong>no</strong>rmal placentas with that of placentasfrom complicated pregnancies.TF% TFPI% TFPI-2% PAI-2 TXA2R a TXA2R bMean SD Mean SD Mean SD Mean SD Mean SD Mean SDN 105 8,2 112,5 5,8 135 15,1 92 5,8 111 3,7 75 3,1A 105,3 25,6 114 2,6 136 4,7 93 1,8 86,4 4,4 76 3,6B 105,6 4,2 112,5 2,9 134 7,6 94,5 3,1 86,3 4 79 2,6Legenda: N: zone corresponding to the inserction of umbilical cord; A: zone next to N, B: marginalzone of placenta.PO-003PPAR-γ EXPRESSION AND MODULATION IN HUMANSTIMULATED FIBROBLASTSEvangelisti L, Giusti B, Bandinelli B, Attanasio M,Lapini I, Lucarini L, Cesari F, Filoni C, Ferrini S,Abbate R, Gensini GF, Pepe GDipartimento di Area Critica Medico Chirurgica,Università degli Studi di Firenze, ItalyPeroxisome proliferator-activated receptors-γ(PPAR-γ) is a nuclear transcription factor that isinvolved in inflammatory process by modulating theproduction of pro-inflammatory cytokines. Severalstudies have demonstrated that PPAR-gamma isexpressed by different human cells (adipose, endothelialand smooth muscle cells) and displays antiinflammatoryproperties. Recently, in rat si<strong>no</strong>vialfibroblasts, the expression of PPAR-γ and its LPSinducedreduction were demonstrated at both mRNAand protein levels. Data about PPAR-γ expression byhuman fibroblasts are <strong>no</strong>t available. Aim of this studywas to investigate whether PPAR-γ is expressed byhuman skin and adventitial fibroblasts, and to evaluatethe modulation of PPAR-γ expression by differentinflammatory stimuli. For this purpose, we grewdermal fibroblasts from three healthy subjects andfibroblasts from the adventitia of three patientsaffected by abdominal aortic aneurysm. Fibroblastsfrom skin biopsy of controls and from adventitia ofpatients were cultured and incubated with differentinflammatory stimuli (LPS 10 µg/mL; TNF α 50 ng/mL+ IFN-γ 300 U/mL) and incubation times (0h, 6h,12h, 24h). PPAR-γ mRNA from fibroblasts wasassayed by a semi-quantitative RT-PCR method. TheRT-PCR products were quantitated after gel electrophoresisby using the GAPDH mRNA as internalcontrol. Time-course experiments with LPS and TNFα/IFN-γstimuli carried out on adventitial fibroblastsshowed an increase of PPAR-gamma expression at 6,12 and 24 hours respectively; also stimulated dermalfibroblasts displayed an increased PPAR-γ expression,but of mi<strong>no</strong>r intensity than adventitial fibroblasts.At the best of our k<strong>no</strong>wledge, this is the firsthaematologica vol. <strong>89</strong>(suppl. n. 8):september <strong>2004</strong>
96Postersreport showing the presence of PPAR-γ expression byhuman dermal and adventitial fibroblasts. Our dataindicate that PPAR-gamma expression is modulatedby inflammatory stimuli and that the fibroblasts fromadventitia react more intensively.PO-004ANTITHROMBOTIC AND ANTIIFLAMMATORY PROPERTIES OFROSUVASTAIN IN CULTURED HUMAN ENDOTHELIAS CELLSMussoni L, # Brambilla M, # Banas A, # Eligini S, #Camera M,* # Tremoli E, # * Colli S ##Dipartimento di Scienze Farmacologiche,Università degli Studi di Mila<strong>no</strong>, Milan, *CentroCardiologico Monzi<strong>no</strong>, IRCCS, Milan, ItalyRosuvastatin (R) is a new 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMG-CoA) inhibitorwith distinct physicochemical, pharmacokinetic andpharmacologic properties; it is relatively hydrophilic,with a plasma half-life of about 20 h, and, besidesplasma LDL-cholesterol level reduction, it lowerstriglycerides and increases HDL-cholesterol inpatients with mixed dyslipidemia or hypertriglyceridemia.Statins, independently of their lipid loweringeffect, modulate several mechanisms that areinvolved in the atherothrombotic process. In the presentstudy we evaluated the effect of rosuvastatin onPAI-1 and tissue factor (TF) in cultured human umbilicalvein endothelial cells (HUVEC). Moreover, thecapacity of the drug to control inflammation wasassessed through the evaluation of eNOS and Cox-2levels. Rosuvastatin, concentration-dependently (10-100 µM) reduced PAI-1 antigen, either secreted orstored, in resting HUVEC (10 µM R: -68%, p< 0.01).In contrast, TF activity induced by TNFα was scarcelyaffected, even at the highest concentration used(100 µM). eNOS levels, detected by Western blotanalysis, were increased by rosuvastatin in a concentration-dependentfashion (25 µM R: +76%,p
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