Haematologica 2004;89: supplement no. 8 - Supplements ...

86Oral CommunicationsTF expression the cells were incubated in the presenceof an anti-AngII antibody. In this experimentalsetting, a strong inhibition of TF activity could beobserved. These results could, at least in part, explainthe efficacy of ACE inhibitors and AT1 receptorantagonists in some types of malignancy, and furthersupport their use for tumor control.CO-127LOW-MOLECULAR WEIGHT HEPARINS REDUCE THEFUNCTIONAL ADHESION OF DIFFERENT TUMOR CELLTYPES TO THE VASCULAR ENDOTHELIUMVignoli A, Marchetti M, Agosti M, Falanga ADepartment of Hematology, Ospedali Riuniti,Bergamo, ItalyAdhesion to the vascular wall is an important stepfor tumor cell extravasation and distant metastasisformation. In this study we evaluated the effect oftwo LMWHs (i.e.: dalteparin, DLT, and enoxaparin,ENX) and unfractionated heparin (UFH) on the functionaladhesion of tumor cells to human endothelialcells (EC) of two types: the microvascular cell lineHMEC-1, and the macrovascular HUVEC. After 24hincubation of EC monolayer with each heparin (10IU/mL) ± IL-1β (5 ng/mL), or the vehicle (control cells,C), the adhesion of two human tumor cell lines (i.e.the leukemic NB4 and the breast cancer MDA-MB-231 cell lines) was analyzed. Simultaneously, quantificationof the main EC surface adhesion molecules(ICAM-1, VCAM-1, E-selectin) by flow cytometry andby ELISA was performed. Tumor cell adhesion to ECmatrix produced by heparin-treated EC was also evaluated.All heparins significantly (p