Vaccines-2013 - OMICS Group

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Jia-hui Lei et al., J Vaccines Vaccin 2013, 4:5http://dx.doi.org/10.4172/2157-7560.S1.0173 rd International Conference onVaccines & VaccinationJuly 29-31, 2013 Embassy Suites Las Vegas, NV, USAEffect of Trichinella spiralis infection on the immune response to HBV vaccine in a mouse modelJia-hui Lei, Fei Guan, Xiao Hou, Wangfang Jiang and Wen-qi LiuDepartment of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, P. R. ChinaVaccination is the most effective and cost-saving way to hepatitis B virus (HBV) infection. Collective data suggest that helminthinfections affect immune responses to some vaccines. Therefore it is important to reveal the effects of helminth infectionson protective vaccines efficacy in countries with highly prevalent helminth infections. In the present work, effects of Trichinellaspiralis infection on the protective efficacy of HBV vaccine in a mouse model were investigated. This study demonstrated thatthe enteric stage of T. spiralis infection could inhibit the proliferative response of spleen lymphocytes to hepatitis surface antigen(HBsAg) and lead to lower levels of anti-HBsAg antibodies, IFN-γ and IL-2, along with higher levels of IL-4 and IL-5. However,these immunological differences are absent in the muscle stage of T. spiralis infection. The results suggest that the muscle stage ofT. spiralis infection does not affect the immune response to HBV vaccination, while the enteric stage infection results in a reducedimmune response to HBsAg.Keywords: Trichinella spiralis; protective efficacy; hepatitis B; vaccineleijiahui@ hust.edu.cnJ Vaccines Vaccin 2013ISSN: 2157-7560, JVV an open access journalVaccines-2013July 29-31, 2013Volume 4 Issue 5Page 110
Byung Chul Kim et al., J Vaccines Vaccin 2013, 4:5http://dx.doi.org/10.4172/2157-7560.S1.0173 rd International Conference onVaccines & VaccinationJuly 29-31, 2013 Embassy Suites Las Vegas, NV, USAEstablishment of the 3 rd national standard for in vitro potency assay of Japanese encephalitisvirus vaccineByung Chul Kim, Hyung-sil Moon, DoKeun Kim, Tae Moo Yoo, Sung Hwa Hong, Naery Lee, Jong-Mi Lim, Donghee Kim, Seokkee Chang,Jiyoung Hong, Jooyeon Lee and Ho Jung OhNational Institute of Food and Drug Safety Evaluation, KoreaTraditionally, the quality control for Japanese Encephalitis Virus (JEV) vaccine has performed in vivo potency assay usinganimals. The Ministry of Food and Drug Safety (MFDS) established alternative in vitro assay (ELISA) replacing the in vivoassay requiring animals and many times as an official quality control method of potency test for the JEV vaccine. The in vitropotency assay showed it's faster and easy to perform without pre-treatment such as a mouse immunization. Also it had betterprecision and reproducibility comparing to the conventional in vivo assay.The reference material is essential in order to evaluate potency test for the JEV vaccine. The 1 st and 2 nd national standardfor in vivo potency assay of JEV vaccine had manufactured, each established in 2001 and 2007, and have been using for themanufacturer's quality control and national lot release since then. As the need of the national standard for in vitro potency assay,this study was initiated by MFDS in 2013 to manufacture and establish the 3 rd national standard for in vitro and in vivo potencyassay of JEV vaccine. The in vitro and in vivo potency results of the candidate material for 3 rd national standard, each weremeasured 1.077 and 2.761. In the study hereafter, the collaborative study of the MFDS and manufacturers will be conducted toestimate the reliable virus content with the candidate material.happysea@korea.krJ Vaccines Vaccin 2013ISSN: 2157-7560, JVV an open access journalVaccines-2013July 29-31, 2013Volume 4 Issue 5Page 111
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