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Vaccines-2013 - OMICS Group

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Pirouz Daftarian, J <strong>Vaccines</strong> Vaccin <strong>2013</strong>, 4:5http://dx.doi.org/10.4172/2157-7560.S1.0163 rd International Conference on<strong>Vaccines</strong> & VaccinationJuly 29-31, <strong>2013</strong> Embassy Suites Las Vegas, NV, USAYour vaccine is served; BON APPETIT: An oral DNA carrying nanovaccine targets APCand eradicates melanoma in micePirouz DaftarianUniversity of Miami, USACurrently, one the most promising vaccine strategy that shows promise in treating cancers consists of personalized dendriticcells (DCs) loaded with antigens. The universal benefits of such cell based vaccines are limited as they are costly and requirespecialized techniques. Here, we present the use of a nanocarrier that carries antigens and targets predominantly DCs, in vivo, hasintrinsic immunopotentiating effects, and works orally. The platform, peptide-derivatized-dendrimer (PDD) where the coupledpeptide is an MHC class II ligand to home onto professional antigen presenting cells (APC). PDD has a positive net charge andtherefore forms a complex with antigens (protein or DNA plasmids). Furthermore, since a universal T helper epitope is tailoredas APC recognition peptide, PDD significantly enhances immune responses. The built-in universal T epitope has two roles inPDD, i) targets the flank of MHC class II of and ii) activate the immune system of >95% of the human population, negatingMHC restriction. Analysis of peritoneal cells and of the draining lymph nodes upon injection of PDD/GFP-DNA complex inmice resulted in GFP expression in >80 of APC. Injection of PDD/TRP2 DNA (plasmids harboring TRP2 complexed with PDD)resulted in the rejection of established B16 melanoma tumors. Next, the oral enteric coated PDD formulation was optimized andone administration of oral formulation of PDD/OVA-DNA could reject OVA/B16 melanoma tumors in 100% of vaccinated mice.Interestingly, oral PDD elicited strong T cell and humoral responses upon a single oral gavage.pdaftarian@med.miami.eduJ <strong>Vaccines</strong> Vaccin <strong>2013</strong>ISSN: 2157-7560, JVV an open access journal<strong>Vaccines</strong>-<strong>2013</strong>July 29-31, <strong>2013</strong>Volume 4 Issue 5Page 72

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