LABOULBENIALES - Agentschap voor Natuur en Bos

Screening of the insects was done with a stereomicroscope at 50x. Thalli wereremoved and mounted on permanent slides following the protocol fromBENJAMIN (1971) and DE KESEL (1998). The latter uses the AraGly embeddingmedium, composed of:5 mL lactic acid;60 mL aq.dist;35 g purified Arabic gum;30 mL glycerin;5 g phenol chrystals;0,1 g cotton blue.Upon each slide, a thin layer medium was placed. The thalli were positioned inthe medium, and given some time (minutes) to dry. Hereafter, a drop of theAraGly medium was placed upon the thalli, followed by the cover slips. Thisway, the briefly fixed-dried Laboulbeniales remained better in place whilefinishing the slide. The microscope slide collection (numbers ending with –a) isdeposited at the Herbarium of the National Botanic Garden of Belgium;numbers ending with –b and –c are kept at respectively the Herbarium ofNatuurmuseum Brabant (Tilburg, The Netherlands) and the private collection ofthe author (Chantemerle-lès-Grignan, France). All infected insects are kept atNatuurmuseum Brabant (Tilburg, The Netherlands).Photographs of intact specimens were taken some 24 hours after mountingusing an Olympus BX51 light microscope with digital camera and AnalySIS Fiveimaging software (Soft Imaging System GmbH). Measurements were takenfrom calibrated photographs of adult thalli; i.e. thallus length, receptaclelength, perithecium length and width and length of inner/outer appendages.2.2.2. IDENTIFICATI ON OF LABOULBENIALESPreparation and identification of the specimens was done by André De Kesel.Identification of the thalli was done up to species level, using appropriateidentification keys and/or original descriptions:DAINAT et al. (1974) : Identification of Stigmatomyces;DE KESEL (1998): Identification of Laboulbenia;DE KESEL (2002): Identification of Rhachomyces;MAJEWSKI (1994);SANTAMARIA (1998): Identification of Laboulbenia;SANTAMARIA (2003): Identification of Haplomyces and Hesperomyces;THAXTER (1896): Identification of Haplomyces.2.3. PRESENTING RESULTSBoth a parasite-host and a host-parasite list are given. In the parasite-host listall hosts are recorded under the parasite. These have been arranged inalphabetical order. In the host-parasite list all recorded hosts are arranged ina systematic order. The nomenclature of hosts follows VORST (2010)(Carabidae, Staphylinidae, Coccinellidae).P a g e | 84