Shodex Catalogue 2013-2015.pdf - Analytics Shop

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Standard AnalysisColumnsColumns for Polymer-basedReversed Phase ChromatographyFeaturesODP-50, C8P-50, C4P-50Relative large pore size is suitable for the analysis of amino acids, peptides, and proteinsUsable in a wide pH range from acidic to alkaline (pH 2 to 13)Usable in 100% water or buffer solutionBest used for the analysis of basic substancesColumns for Polymer-based Reversed Phase ChromatographyODP-40Standard columnsProduct CodeF7621001F7621002F7620002F7620001F6710001F6710023F7620004F7620003F6710022F7620009F6713001F7620006Product NameNewsNo.35Higher performance type of ODP-50 seriesNewsAsahipak ODP-40 4DAsahipak ODP-40 4EAsahipak ODP-50 6DAsahipak ODP-50 6EAsahipak ODP-50G 6AAsahipak ODP-50 4BAsahipak ODP-50 4DAsahipak ODP-50 4EAsahipak ODP-50G 4AAsahipak ODP-50 2DAsahipak ODP-50G 2AAsahipak C8P-50 4DNo.1Plate Number(TP/column)≥ 11,000≥ 17,000≥ 9,000≥ 14,000(guard column)≥ 2,500≥ 9,000≥ 14,000(guard column)≥ 5,000(guard column)≥ 7,000FunctionalGroupOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctylParticle Size(µm)445555555555Pore Size(Å)250250250250−250250250−250−250Column Size(mm)I.D. x Length4.6 × 1504.6 × 2506.0 × 1506.0 × 2506.0 × 104.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 1502.0 × 104.6 × 150PreparativeColumnsSemi-microMicro ColumnsShipping SolventH 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65p.78p.72F7620005Asahipak C8P-50 4E≥ 11,000Octyl52504.6 × 250H 2 O/CH 3 CN=35/65F6710002Asahipak C8P-50G 4A(guard column)Octyl5−4.6 × 10H 2 O/CH 3 CN=35/65F7620008Asahipak C4P-50 4D≥ 6,000Butyl52504.6 × 150H 2O/CH 3CN=35/65F7620007Asahipak C4P-50 4E≥ 9,000Butyl52504.6 × 250H 2 O/CH 3 CN=35/65F6710003Asahipak C4P-50G 4A(guard column)Butyl5−4.6 × 10H 2 O/CH 3 CN=35/65Base Material : Polyvinyl alcoholFeatures and applications of different packing materials used for columns for reversed phase,hydrophilic interaction and normal phase chromatographyLargePore size of packing materialsSilicapakSILSaccharides,Sugar alcohols,Hydrophilic low molecularweight substancesDM-614PolycyclicaromaticcompoundsC4P-50C8P-50Peptides5NH 5CN 5C4 5C8 ODSNH2P-40,50ODP2 HPDEProteinsODP-40,50RP18-415DS-413,613VitaminsSmallLow molecular weight organic compounds,drugs, organic acids, food additives, etc.08HighPolarity of packing materialsLow*Contact Shodex or our distributors near you for customized columns.
AlkylalcoholsC8C6C4EGC6C10C12C14C8C4EG C10 C12C4P-50C8P-50Elution volume (mL)15105Sample : Ethyleneglycol, n-AlkylalcoholODP-50C8P-50C4P-50Tolerance of ODP-50 for alkaline condition123Sample :1. Acetophenone2. Butyrophenone3. Hexanophenone4. Heptanophenone5. Octanophenone45330hrStandard AnalysisColumnsC6 C4EG0 5231C8C10Local anesthetics0 20 minC14ODP-50C12C1410 15 20 25 minpH3 pH7 pH111230 20 minColumn : Shodex AsahipakODP-50 4DEluent : 25mM Phosphate buffer/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚C2 4 6 8 10 12 14Carbon number (n)Relationship between the carbon number ofalkylalcohols and elution volumeColumn : Shodex Asahipak ODP-50 4DAsahipak C8P-50 4DAsahipak C4P-50 4DEluent : H 2 O/CH 3 OH=20/80Flow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CDissociation of tertiary amino groups in basic drugs can be suppressed by making pH of the eluenthigher than pKa of the amino groups. This increases the relative hydrophobicity of the basic drugs,thereby allowing the column to retain the drugs stronger and provide baseline separation of them.10 20 min23Sample :1. BenzocaineH2N2. LidocaineCH3CH33. TetracaineNHCOCH2NCOOCH2CH2NNH(CH2)3CH3COOC2H5C2H5C2H5CH3CH30 10 20 min120hr90hrSample : 20μL1. Heptaoxyethylenedodecylether(PAR-Co) 1μg/L2. PAR230hrColumn : Shodex Asahipak ODP-50 4DEluent : 10mM NaOH aq.(pH12.0)/CH 3 CN=35/65Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CNonionic surfactants0 1 2 3 4 5 6 7minColumn : Shodex Asahipak ODP-40 4DEluent : 10mM Sodium tetraborate aq./CH 3 OH=62/38Flow rate : 0.8mL/minDetector : VIS(510nm)Column temp. : 40˚CColumns for Polymer-based Reversed Phase ChromatographyFat-soluble vitamins Macrolide antibiotics Gradient analysis of proteins and peptides21Sample : 20μL1. Vitamin K3 1.5μg/mL2. Vitamin A 1.0 IU/mL3. Vitamin A acetate 0.5 IU/mL4. Vitamin D2 13.2μg/mL5. Vitamin D3 13.2 IU/mL6. Vitamin E acetate 2.4μg/mL7. Vitamin E 2.5μg/mL8. Vitamin K1 2.4μg/mL5481Sample : 0.1% each, 10μL1. Erythromycin2. Azithromycin25642 3120%CH3CN891071112Sample : 40μL12345678950%CH3CNSample Mw Recovery(%)Lys-Bradykinin 1188 97Bradykinin 1060 92Met-Enkephalin 574 97Neurotensin 1673 99Leu-Enkephalin 556 100Substance P 1348 93Bacitracin 1450 81Insulin5750 95Insulin B chain 3496 913671011LysozymeMastoparan143001479969612Myoglobin175008305 10 15 20 25min0 10 20 min0 510 15 20 25minColumn : Shodex Asahipak ODP-50 4EEluent : CH 3 CN/CH 3 OH=50/50Flow rate : 0.6mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ODP-40 4EEluent : 40mM Potassium phosphatebuffer(pH11.0)/CH 3 CN=40/60Flow rate : 0.5mL/minDetector : UV(223nm)Column temp. : 40˚CColumn : Shodex Asahipak ODP-50 6DEluent : (A); 0.05% TFA aq./CH 3 CN=80/20(B); 0.05% TFA aq./CH 3 CN=50/50Linear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 30˚C09
Page 2: TMWe provide a wide range of produc
Page 7 and 8: BiotechnologyGenomicsProteomicsGlyc
Page 9: Comparison between ODP2 HP and ODP-
Page 13 and 14: Separation and recovery rate of sta
Page 15 and 16: Fructooligosaccharide syrup2Sample
Page 17 and 18: Hippuric acids in urineStandards2Sa
Page 20 and 21: Standard AnalysisColumnsColumns for
Page 28 and 29: Standard AnalysisColumnsColumn Sele
Page 30 and 31: Standard AnalysisColumnsAqueous SEC
Page 32 and 33: Standard AnalysisColumnsAqueous SEC
Page 34 and 35: Standard AnalysisColumnsMultimode C
Page 36 and 37: Standard AnalysisColumnsAqueous/Org
Page 38 and 39: Standard AnalysisColumnsOrganic SEC
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Standard AnalysisColumnsColumns for
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Standard AnalysisColumnsLC/MS analy
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Sample PretreatmentColumnsColumns f
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Sample PretreatmentColumnsPretreatm
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Application FieldsPharmaceuticals a
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Application FieldsBiotechnology Ana
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Application FieldsWater quality[Ani
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Semi-micro andMicro ColumnsSemi-mic
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PreparativeColumnsPreparativeColumn
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InformationPhase-out ProductsPhase-
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InformationColumn Cleaning Procedur
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InformationColumn Trouble ShootingC
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IndexIndex by Product NameColumns a
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Technical InformationTechnical Note
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Please contact a Shodex support off
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