Shodex Catalogue 2013-2015.pdf - Analytics Shop

HPLC System Trouble ShootingCommon causes for abnormal chromatogramsInformationUnstable baseline valuesSTART······Main cause······Solution······Frequent problemColumn PressurenormalYESBaseline stable whenpump stoppedYESNONOYESMonotonic driftNOMalfunction of check valveTemperature fluctuationDeterioration of detector lampInsufficient groundingMalfunction of recorderBubbles in flow cell· Replace check valve· Clean up check valve· Warm up detector sufficiently· Shield detector from wind· Check column oven· Check detector temperature controlReplace lampEstablish sufficient groundingRepair recorderWash inside of detectorHPLC System Trouble ShootingContamination of flow cellWash inside of detectorLeakage observedNOInsufficient eluent replacementWash flow line of HPLCYES(with different solvent, salt or others)Contamination of column· Wash column· Check column performance· Replace columnFlow rate fluctuationPulsation of pumpUse damperTighten connectionsDeterioration of eluent· Use eluent containing stabilizer(for THF, use THF containing BHT)Noise······Main cause······Solution······Frequent problemSTARTInsufficient groundingEstablish sufficient groundingBaseline stable whenpump stoppedNOIrregular noiseDeterioration of detector lampReplace detector lampYESMalfunction of recorderRepair recorderShodex DEGAS usedNOInsufficient degassing eluentUse Shodex DEGASYESSpike noiseYESOKSpike noise foundfrom beginingNOYESAir in columnProblem solved by applying backpressure to column(*)(*) Do not apply back pressure to detectorColumn temperature exceeding eluent boiling pointNO· Check column performance· Replace columnReduce temperature of columnoven and/or post-column reactioncoilNoise shape leadingNOContamination of flow cellWash flow cellYESBubbles in flow cellRepair detector89