Shodex Catalogue 2013-2015.pdf - Analytics Shop

Protein separation using cation exchange columns( I ) CM-825(Weak cation exchange)90µL injected10 15 30 45 min123423546HOHO1243560 15 30 min 0 2 4 6 minColumn : ( I ) Shodex IEC CM-825, ( II ) IEC SP-825, ( III ) IEC SP-420NEluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl( I , II ) Linear gradient; (A) to (B), 60min ( III ) Linear gradient; (A) to (B), 10minFlow rate : ( I , II )1.0mL/min ( III )1.5mL/minDetector : UV(280nm)Column temp. : Room temp.Sample : 300μg/mL each, 10μLCH 2·CH·NH 2COOH2. AdrenalineHO CH·CH 2·NHHO OH CH 33. NoradrenalineHO CH·CH 2·NH 2HOOH12123456Sample :1. Myoglobin2. Trypsinogen3. Ribonuclease A4. α-Chymotrypsinogen A5. Cytochrome c6. LysozymeRapid analysis of proteins using UHPLCCatecholamines Angiotensins Paraquat and diquat1. DOPA( II ) SP-825(Strong cation exchange)30µL injected( III ) SP-420N(Strong cation exchange)non-porous type gelSample : 10μL1. Angiotensin II (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe2. Angiotensin I (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe-His-Leu3. Angiotensin III (human)Arg-Val-Tyr-lle-His-Pro-Phe10.02345Sample : 5μL (13mg total protein )1. Myoglobin2. Trypsinogen3. Ribonuclease A4. α-Chymotrypsinogen A5. Cytochrome c6. Lysozyme61.0 2.0 3.0 4.0minColumn : Shodex PIKESS SP-2BEluent : (A); 20mM Sodium phosphatebuffer(pH 7.0)(B); (A) + 0.5 M NaClLinear gradient; 100%(A) to 50%(B),2.5minFlow rate : 1.2mL/minDetector : UV(280nm)Column temp. : 25˚CParaquatSample : 20μL+ +H 3 C N N CH 3 2Cl - Diquat+ 2Br -N + NStandard AnalysisColumnsColumns for Cation Exchange Chromatography4. DopamineHOHOCH 2·CH 2·NH 230 20 min0 10 20 30 min0 10 20 minColumn : Shodex Asahipak ES-502C 7CEluent : 20mM Sodium malonate buffer+ 0.5M NaCl(pH6.0)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium malonate buffer(pH6.0)/CH 3 CN=80/20Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium phosphate buffer+ 150mM NaCl(pH7.0)Flow rate : 1.0mL/minDetector : UV(288nm)Column temp. : 30˚CAnalysis of pochonicine and their analogues in filamentousfungi culture extractStandard amino acidsSample : 5μLFraction purified from the culture extract ofPochonia suchlasporia var. suchlasporia TAMA 87(Fungi culture provided by Dr. Toru Okuda,Tamagawa University Research Institute)CysSample : 2.5nmol each, 100μLThrSerGluAspGlyValMetIleLysAlaHisPochonicineLueTyrPheNH3ArgAnalogue AAnalogue BPro0 5 10 15 20 25 30 35 minColumn : Shodex Asahipak ES-502C 7CEluent : 10mM Ammonium bicarbonate aq.Flow rate : 0.6mL/minDetector : UV(210nm)Column temp. : 40˚CData provided by Dr. Teruhiko Nitoda, Faculty of Agriculture, Okayama University.01020 30 40 50 minColumn : Shodex CXpak P-421SEluent : No.1 0.12M Sodium citrate buffer(pH3.3)No.2 0.13M Sodium citrate buffer(pH3.2)No.3 0.11M Sodium citrate buffer(pH4.0)No.4 1.02M Sodium citrate buffer(pH4.9)No.5 0.2M NaOH aq./Ethanol=90/10 (Rinse solution)Low pressure gradient;No.1 (0min), No.2 (1.2min), No.3 (10.0min), No.4 (21.2min), No.5 (40min)Flow rate : 0.5mL/minDetector : VIS(570nm)Post column method(Ninhydrin reaction 0.35mL/min, 120˚C)Column temp. : 63˚C55