Shodex Catalogue 2013-2015.pdf - Analytics Shop

Calibration curves for GF-HQ series usingpullulanMolecular weight (Pullulan)10 7 Elution volume (mL)10 610 510 410 3GF-210 HQGF-510 HQGF-710 HQGF-310 HQGF-7M HQCalibration curves for GF-HQ series usingPEG/PEOMolecular weight (PEG/PEO)10 7 Elution volume (mL)10 610 510 410 3GF-510 HQGF-210 HQGF-310 HQGF-710 HQGF-7M HQPolyacrylonitrileSample : Polyacrylonitrile 0.1%(w/v), 100μLStandard AnalysisColumns10 2103 4 5 6 7 8 9 10 11Column : Shodex Asahipak GF-HQ seriesEluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CComparison of polystyrenes separation using various solvents3 4Sample : Polystyrene 1mg/mL each, 50μL1. Mw : 1,090,0002. Mw : 190,0003. Mw : 37,9004. Mw : 9,1005. Mw : 2,9803245 6 5 61 6. Mw : 500(A) THF(B) Chloroform (C) DMF1123245610 2103 4 5 6 7 8 9 10 11Column : Shodex Asahipak GF-HQ seriesEluent : DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CSodium polystyrene sulfonatesMW:104,0000 10 20 30 minMW:38,0000 10 20 min0 5 10 15 20 25 30 35 40minColumn : Shodex Asahipak GF-710 HQ x 2Eluent : 20mM LiBr in DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CPolymers having both hydrophobicand hydrophilic functional groupsmay exhibit hydrophobic interactionswith packing materials.When analyzing such polymers, thehydrophobic interaction can besuppressed by adding organicsolvents to the eluent.Sample : 1mg/mL each, 50μLSodium polystylene sulfonateCH2CHSO3NanAqueous/Organic SEC Columns0 20 min 0 20 min 0 20 minColumn : Shodex Asahipak GF-510 HQ + GF-310 HQEluent : (A); THF, (B); Chloroform, (C); DMFFlow rate : 0.5mL/minDetector : (A),(B) UV(254nm), (C) UV(270nm)Column temp. : 30˚C0MW:8,00010 20 30 minColumn : Shodex AsahipakGF-510 HQEluent : 50mM LiCl aq./CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CComparison of two GF columns for the separation performanceof maltooligosaccharidesComparison of two GF columns for the separation performanceof triglyceridesGF-210 HQ demonstrates an improved separation of low molecularsubstances. Below example shows that the peaks obtained by GF-210 HQ areseparated with deeper notches compared to peaks obtained by GF-310 HQ.GF-210 HQ is capable of separating oligosaccharides (trisaccharides tohexasaccharides) while those oligosaccharides were eluted all together whenanalyzed by GF-310 HQ.GF-210 HQ x 2213456Sample : 0.1% each, 20μL1. Maltohexaose2. Maltopentaose3. Maltotetraose4. Maltotriose5. Maltose6. GlucoseSample : 50μL1. Tristearin 0.1%2. Tricaprin 0.2%3. Tricaproin 0.2%4. Tributyrin 1.0%GF-210 HQ212431~344356GF-310 HQ1GF-310 HQ x 21520 25 30 35 min0 5 10 15 minColumn : Shodex Asahipak GF-210 HQ x 2, GF-310 HQ x 2Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 50˚CColumn : Shodex Asahipak GF-210 HQ, GF-310 HQEluent : ChloroformFlow rate : 0.6mL/minDetector : RIColumn temp. : 50˚C35