Shodex Catalogue 2013-2015.pdf - Analytics Shop

InformationColumn Trouble ShootingCommon causes for abnormal chromatogramsUnstable baseline values······Main cause······Solution······Frequent problemColumn Trouble ShootingPeak tailingPeak split,shouldersPeakleadingBroad peakSelection ofcolumn and/or eluentappropriateProblem solvedby reducinginjection volumeNOYESNOYESStrong sample retentionVoid existing at top of columnAdsorption of impurities incolumnChanneling in columnLeading caused by sampleproperties(ionic and micellar properties)· Adsorption of impurities incolumn· Deterioration of columnSample overloadChange column and/or eluentReplace column· Wash column· Check column performance· Replace columnReplace columnChange column and/or eluent· Wash column· Check column performance· Replace columnReduce injection volumeGhost peakContaminationImpurities existing in eluentPrepare sample againWash flow line of HPLCReplace eluentNo peakSystem trouble· Eluent not flowing· Injector at wrong position (load/inject)· Detector deactivatedCheck systemSample adsorption in columnRepeat sample injection· Column equilibration insufficientPressure elevationPumpA B CColumn Detector······Main causeSTARTInjector······Solution······Frequent problemSalt precipitated isobserved in eluentYESPressure still highafter washingNOSalting out· Change eluent composition· Wash system before analysisNOYESPressure still high whenC is disconnectedNODetector pipingblocked· Clean up detector· Replace pipingYESPressure still high whenB is disconnectedYESNOColumnblockedPressure undermaximum columnpressureYESNOScreen eluent and samplethrough 0.45 μm filterYESNODeteriorationof columnColumn end-fittingblocked· Check column performance· Replace columnReplace columnPressure still high whenA is disconnectedNOInjector blocked· Clean up injector· Replace injectorYESUsed line filterYESNOPump piping blocked· Replace filter in pump· Clean up pump· Replace pump piping88Line filter blocked· Wash line filter· Replace line filter