Shodex Catalogue 2013-2015.pdf - Analytics Shop

Standard AnalysisColumnsLC/MS analysis of N-acetyl-chitooligosaccharidesN-Acetyl-D-glucosamine2merSample : 5μLMixture of N-acetyl-chitooligosaccharides 500ng/mL3merm/z 220m/z 423m/z 626LC/TOF-MS analysis of 2-amino benzoic acid derivatizedsugar chains1234 5Sample : 2μLHuman IgG (1μg protein)2AA-labeled N-Glycan* 2AA : 2-Aminobenzoic acidColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns60[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography012NCH3NHCreatinineNH3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 min412114merH2N5mer36mer0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 minSample : 100ng/mL each, 5μL1. Creatinine2. Creatine1O10CH3NNHCreatineLC/MS/MS analysis of estradiol metabolites5OOH26m/z 829m/z 1032m/z 1235Column : Shodex Asahipak NH2P-40 2DEluent : (A); 0.05% Ammonia aq. (B); CH 3 CNLinear gradient; (B%) 75%(0min) 50%(10min) 50%(15min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative)Column temp. : 30˚CLC/MS analysis of creatinine and creatineColumn : Shodex Asahipak NH2P-40 2DEluent : (A); 50mM Ammonium formate aq. (B); CH 3 CNLinear gradient;B%, 90%(0min) 90%(6min) 75%(7min) 75%(20min)Flow rate : 0.2mL/minDetector : Creatinine : ESI-MS, Creatine : ESI-MS/MSColumn temp. : 30˚C7895 10 15 20Migration time (min)Column : Shodex Asahipak NH2P-40 2DEluent : (A); 95% CH 3 CN/0.1% Formic acid aq.(B); 5% CH 3 CN/0.1% Formic acid aq.Linear gradient; (B%) 30%(0-2.5min), 30-95%(2.5-20min)Flow rate : 0.2mL/minDetector : ESI-TOF MS(Polarity : Negative, Full MS range : 2000 )Column temp. : 45˚CData provided by Michihiro Kinoshita Ph.D., Faculty of Pharmacy, Kinki UniversityLC/MS analysis of melamine and cyanuric acid0 5 10 15Time/minData provided by Mr. Atsushi Yamamoto,Osaka City Institute of Public Health and Environmental Sciences0 121H 2N N NH 2NNH2NMelamine3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 min2HOSample : 500ng/mL each, 5μL1. Melamine2. Cyanuric acidNNOHNCyanuric acidColumn : Shodex Asahipak NH2P-40 2DEluent : 50mM Ammonium formate aq./CH 3 CN=25/75Flow rate : 0.2mL/minDetector : Melamine : ESI-MS/MSCyanuric acid : ESI-MSColumn temp. : 30˚CSample : 0.1ppm each, 5μL1. Estrone-3-sulfate2. β-Estradiol-3-sulfate3. Estriol-3-sulfate4. Dehydroepiandrosterone-3-sulfate5. Estrone-3-glucuronide6. β-Estradiol-17-glucuronide7. β-Estradiol-3-glucuronide8. Estriol-3-glucuronide9. Estriol-16-glucuronide10. Dehydroepiandrosterone-3-glucuronide11. Androsterone-3-glucuronideColumn : Shodex Asahipak NH2P-40 2DEluent : (A); 10mM NH 4 OH CH 3 CN(B); 10mM NH 4 OH H 2 OLinear gradient;B%, 22%(0-5min), 22-70%(5-10min),70%(10-15min)Flow rate : 0.2mL/minDetector : ESI-MS/MS(Polarity : Negative)Column temp. : 40˚COH6