Shodex Catalogue 2013-2015.pdf - Analytics Shop

Proteins in human serumSample : Human serum 0.5%, 200μL1. IgG42. Transferrin3. (Unknown)4. Serum albumin01122315 30 45 minColumn : Shodex IEC QA-825Eluent : (A); 20mM Tris-HCl buffer(pH8.6)(B); (A) + 0.5M NaClLinear gradient; 100% (A) to 50% (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.3 3120 5 10 15 20min1343 420 5 10 15 20minComparison of porous DEAE-825 and non-porous DEAE3N-4T for protein separationporous gel DEAE-825Effects of eluent pH for DEAE-825pH6.0pH8.0IEC DEAE3N-4T is a weak anion exchange column, having diethylaminoethyl functional group modified onnon-porous gel. The non-porous gel enables rapid analysis of proteins and peptides. DEAE3N-4T is alsosuitable for the analysis of small-volume samples, as it provides sharp peaks even with small injection volume.: Diethylaminoethyl 1: Diethylaminoethyl21 1,20 5 10 15 20min1324230 5 10 15 20min4HOHOOC1. endo GA14CH3HOHOOCSample : 100μL1. Conalbumin 0.1%2. Transferrin 0.2%3. Ovalbumin 0.2%4. Trypsin inhibitor 0.2%Gibberellin Isomers2. GA14340 5 10 15 20minColumn : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Bis-Tris-HCl buffer(pH7.0)20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)20mM 1,3-Diaminopropane-HCl buffer(pH10.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 25˚Cnon-porous gel DEAE3N-4TpH7.0pH9.0Sample :1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mLpH10.0CH3COOHCOOH12Sample :GibberellinStandard AnalysisColumnsColumns for Anion Exchange Chromatography0 5 10 15 20 25 30 min0 5 minColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 100μL: Shodex IEC DEAE-825: (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 60min: 1.0mL/min: UV(280nm)ColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 20μL: Shodex IEC DEAE3N-4T: (A); 25mM Piperazine-HCl buffer(pH6.0)(B); A + 0.5M NaClLinear gradient; (A) to (B), 10min: 1.5mL/min: UV(280nm)0 10 20 minColumn : Shodex Asahipak ES-502N 7CEluent : Acetic acid/H 2 O/CH 3 OH=0.1/0.4/99.5Flow rate : 1.5mL/minDetector : UV(210nm)Column temp. : 50˚CData was provided by Prof. Yamaguch,Faculty of Agliculture, University of Tokyo.Mercaptoalbumin andnon-mercaptoalbuminNucleotidesSulfide ion and cyanide ion1 23Sample : Normal control serum1. Globulin2. Uric acid3. HMA(Human Mercaptoalbumin)4. HNA(Human Nonmercaptoalbumin)12467Sample : 20μL1. 5'CMP 20μg/mL2. 5'UMP 20μg/mL3. 5'TMP 20μg/mL4. 3'CMP 20μg/mL5. 5'ADP 100μg/mL6. 3'UMP 20μg/mL7. 5'GMP 20μg/mL8. 2'AMP 20μg/mL9. 3'AMP 20μg/mL10. 3'GMP 20μg/mL89Sample : 20μL1. HS - 0.1mg/L2. CN - 0.02mg/L12435100.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0min0 816 24 minColumn : Shodex Asahipak ES-502N 7CEluent : 50mM N-methylpiperazine-HClbuffer(pH4.8) + 400mM Na 2 SO 4 aq.+ 0.3% C 2 H 5 OHFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 35˚C0 5 10 15 20 25 30minColumn : Shodex AXpak WA-624Eluent : 0.35M CH 3 COOH aq./0.35M CH 3 COONH 4 aq.=240/100Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 60˚CColumn : Shodex IEC DEAE-825Eluent : 10mM Na 2 CO 3+ 1mM Ethylenediamine aq.+ 10% CH 3 OHFlow rate : 1.0mL/minDetector : Electrochemical(Electrode; Silver, 0mV SCE)Column temp. : 25˚C53