Shodex Catalogue 2013-2015.pdf - Analytics Shop

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Standard AnalysisColumnsColumns for Anion Exchange ChromatographyFeaturesQA-825,DEAE-825Suitable for the analysis of relatively high molecular weight compounds,such as proteins, peptides, DNA, and RNAUsable under wide pH range between 2 and 12NotebookNo.7Semi-microMicro ColumnsPreparativeColumnsp.77p.80Columns for Anion Exchange ChromatographyDEAE3N-4TDEAE-2BES-502N 7CWA-624Non-porous base materialFor rapid analysisNotebookNo.6NewsNo.33Non-porous base materialSupports UHPLC (available under hyperbaric conditions up to 30 MPa)NewsNo.42Compared to IEC series columns, polyvinyl alcohol is used as base materialand this offers different separation patternLow hydrophobic interaction of proteins allows analysis under mild conditionsSuitable for anion exchange analysis of low molecular weight compoundssuch as nucleotidesSemi-microMicro ColumnsPreparativeColumnsp.77p.80Standard columnsStrong anion exchange resin (Functional Group : Quaternary ammonium)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6110011 IEC QA-825 0.45 Polyhydroxymethacrylate 12 5,000 8.0 × 75 50mM Na 2 SO 4 aq.Weak anion exchange resin (Functional Group : Diethylaminoethyl)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6118255IEC DEAE-8250.6Polyhydroxymethacrylate85,0008.0 × 7550mM Na 2 SO 4 aq.F6112100IEC DEAE3N-4T0.4Polyhydroxymethacrylate2.5−4.6 × 35H 2 OF6112110PIKESS DEAE-2B0.4Polyhydroxymethacrylate2.5−2.0 × 50H 2 OF7640002Asahipak ES-502N 7C0.55Polyvinyl alcohol92,0007.5 × 10050mM 1,3-Diaminopropane+ 50mM NaCl(pH10.0)F6356240AXpak WA-6241.2Polyhydroxymethacrylate102,0006.0 × 1500.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/20F6700245AXpak WA-G(guard column)Polyhydroxymethacrylate10−4.6 × 100.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/2052*Contact Shodex or our distributors near you for customized columns.
Proteins in human serumSample : Human serum 0.5%, 200μL1. IgG42. Transferrin3. (Unknown)4. Serum albumin01122315 30 45 minColumn : Shodex IEC QA-825Eluent : (A); 20mM Tris-HCl buffer(pH8.6)(B); (A) + 0.5M NaClLinear gradient; 100% (A) to 50% (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.3 3120 5 10 15 20min1343 420 5 10 15 20minComparison of porous DEAE-825 and non-porous DEAE3N-4T for protein separationporous gel DEAE-825Effects of eluent pH for DEAE-825pH6.0pH8.0IEC DEAE3N-4T is a weak anion exchange column, having diethylaminoethyl functional group modified onnon-porous gel. The non-porous gel enables rapid analysis of proteins and peptides. DEAE3N-4T is alsosuitable for the analysis of small-volume samples, as it provides sharp peaks even with small injection volume.: Diethylaminoethyl 1: Diethylaminoethyl21 1,20 5 10 15 20min1324230 5 10 15 20min4HOHOOC1. endo GA14CH3HOHOOCSample : 100μL1. Conalbumin 0.1%2. Transferrin 0.2%3. Ovalbumin 0.2%4. Trypsin inhibitor 0.2%Gibberellin Isomers2. GA14340 5 10 15 20minColumn : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Bis-Tris-HCl buffer(pH7.0)20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)20mM 1,3-Diaminopropane-HCl buffer(pH10.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 25˚Cnon-porous gel DEAE3N-4TpH7.0pH9.0Sample :1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mLpH10.0CH3COOHCOOH12Sample :GibberellinStandard AnalysisColumnsColumns for Anion Exchange Chromatography0 5 10 15 20 25 30 min0 5 minColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 100μL: Shodex IEC DEAE-825: (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 60min: 1.0mL/min: UV(280nm)ColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 20μL: Shodex IEC DEAE3N-4T: (A); 25mM Piperazine-HCl buffer(pH6.0)(B); A + 0.5M NaClLinear gradient; (A) to (B), 10min: 1.5mL/min: UV(280nm)0 10 20 minColumn : Shodex Asahipak ES-502N 7CEluent : Acetic acid/H 2 O/CH 3 OH=0.1/0.4/99.5Flow rate : 1.5mL/minDetector : UV(210nm)Column temp. : 50˚CData was provided by Prof. Yamaguch,Faculty of Agliculture, University of Tokyo.Mercaptoalbumin andnon-mercaptoalbuminNucleotidesSulfide ion and cyanide ion1 23Sample : Normal control serum1. Globulin2. Uric acid3. HMA(Human Mercaptoalbumin)4. HNA(Human Nonmercaptoalbumin)12467Sample : 20μL1. 5'CMP 20μg/mL2. 5'UMP 20μg/mL3. 5'TMP 20μg/mL4. 3'CMP 20μg/mL5. 5'ADP 100μg/mL6. 3'UMP 20μg/mL7. 5'GMP 20μg/mL8. 2'AMP 20μg/mL9. 3'AMP 20μg/mL10. 3'GMP 20μg/mL89Sample : 20μL1. HS - 0.1mg/L2. CN - 0.02mg/L12435100.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0min0 816 24 minColumn : Shodex Asahipak ES-502N 7CEluent : 50mM N-methylpiperazine-HClbuffer(pH4.8) + 400mM Na 2 SO 4 aq.+ 0.3% C 2 H 5 OHFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 35˚C0 5 10 15 20 25 30minColumn : Shodex AXpak WA-624Eluent : 0.35M CH 3 COOH aq./0.35M CH 3 COONH 4 aq.=240/100Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 60˚CColumn : Shodex IEC DEAE-825Eluent : 10mM Na 2 CO 3+ 1mM Ethylenediamine aq.+ 10% CH 3 OHFlow rate : 1.0mL/minDetector : Electrochemical(Electrode; Silver, 0mV SCE)Column temp. : 25˚C53
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Page 11 and 12: AlkylalcoholsC8C6C4EGC6C10C12C14C8C
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Page 15 and 16: Fructooligosaccharide syrup2Sample
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