Shodex Catalogue 2013-2015.pdf - Analytics Shop

Standard AnalysisColumnsColumns for Polymer-based Reversed Phase ChromatographyColumns for Polymer-basedReversed Phase ChromatographyFeaturesODP2 HPStandard columnsProduct CodeF7622001F7622002F7622003F6714010F7622004F7622005F6714011Provides nearly twice as large theoretical plate number compared with generally availablepolymer-based reversed phase columnsColumn offers enhanced retention of high polar substances compared with ODS columnSuitable for reversed phase analysis of small molecules such as pharmaceutical compounds in thepresence of protein matrixIdeal for LC/MS analysis of high polar compoundsNotebook No.6 News No.30, 36See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Product NameODP2 HP-4BODP2 HP-4DODP2 HP-4EODP2 HPG-4AODP2 HP-2BODP2 HP-2DODP2 HPG-2APlate Number(TP/column)≥ 3,500≥ 13,000≥ 17,000(guard column)≥ 3,000≥ 7,000(guard column)FunctionalGroup−−−−−−−Particle Size(µm)5555555Pore Size(Å)404040−4040−Column Size(mm)I.D. x Length4.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 502.0 × 1502.0 × 10Shipping SolventH 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45Base Material : PolyhydroxymethacrylateTolerance of ODP2 HP for alkaline conditionComparison of chromatograms obtained beforeand after passage of alkaline eluentODP2 HP-4DBefore passageAfter 500 hrpassage1 ODS10 5 10min2Before passageAfter 24 hrpassageSample : 5μL1. Pyridine 200μg/mL2. Phenol 430μg/mL0 5 10min2Relative theoretical plate number for pyridineCorrelation between alkaline eluentpassage time and relative theoreticalplate number(%)1201008060402000ODP2 HP-4DODS200 400 600Alkaline eluent passage time (hr)ConditionColumn : Shodex ODP2 HP-4DODS from other manufacturerEluent : H 2 O/CH 3 OH=70/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CEluent passage conditions for alkali tolerance testEluent : 10mM Sodium phosphate buffer(pH12)/CH 3 CN=45/55Flow rate : 0.6mL/minColumn temp. : 30˚C06*Contact Shodex or our distributors near you for customized columns.