Shodex Catalogue 2013-2015.pdf - Analytics Shop

Protein separation by hydrophobicinteraction chromatography01HO23EpinephrinesSample : 370μL1. Cytochrome c 0.03%2. Myoglobin 0.08%3. Ribonuclease A 0.16%4. Ovalbumin 0.16%5. Lysozyme 0.04%6. α-Chymotrypsinogen50.05%6420 40 60 minColumn : Shodex HIC PH-814Eluent : (A); 1.8M Ammonium sulfate + (B)(B); 0.1M Phosphate buffer(pH7.0)Linear gradient; (A) to (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Sample : Epinephrine 50μg/mL, 50μLOHNHOHCH 3121230 510 minColumn : Shodex RSpak DE-413Post column : Shodex AFpak ACH-494Eluent : 0.1M H 3 PO 4 +300mg/L Sodium 1-decansulfonate +65mg/L Tetramethylammonium chloride(pH8.0 adjusted by 1.0M NaOH)Flow rate : 1.0mL/minDetector : Electrochemical(Electrode : Pt, 350mV SCE)Column temp. : 37˚C0 5 10 15 20 25 0 5 10 15 20 25 30 35 0 5 10 15minminminColumn : Shodex ORpak CDBS-453Eluent : 0.05% Acetic acid + 0.2M NaCl aq./CH 3 CN=95/5Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 10˚CMandelic acidsSample : Mandelic acid 100μg/mL, 20μL1. D-Mandelic acid2. L-Mandelic acid0 5 10 15 20 25minCholine and acetylcholineWarfarinSample : Warfarin 20μg/mL, 20μL2 3OOH1 40 1 2 3minOSample : 10μL1. Choline 5mg/L2. Ethylhomocholine 10mg/L3. Acetylcholine 10mg/LAfter choline and acetylcholineare separated using DE-413polymer-based reversedphase column, solutes arepassed through ACH-494 togenerate hydrogen peroxide.The resulting hydrogenperoxide is detected using anelectrochemical detector toenable highly sensitiveanalysis.CHCH2COCH3Column : Shodex ORpak CDBS-453Eluent : 1.0% Acetic acid + 0.2M NaCl aq./CH 3 CN=80/20Flow rate : 0.6mL/minDetector : UV(310nm)Column temp. : 16˚C0* See page 10 for DE-41312Lipoproteins in plasmaLDLEluent A Eluent BHDLEluent A30 10 20 30 min 0 10 20 30 minEluent BSample :Lipoproteins(Sigma)2 4 6 8 10 12 14min1Ligand : Dextran sulfate0 10 20 30 40 minColumn : Shodex AFpak ADS-894Eluent : (A); 50mM Sodium phosphate buffer(pH7.4)(B); (A) + 1.0M NaClStep gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Lactic acidsSample : Lactic acid 50μg/mL, 50μL1. L-Lactic acid2. D-Lactic acidColumn : Shodex ORpak CRX-853Eluent : 0.5mM CuSO 4 aq.Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CSample :1. Uracil2. Pyridine3. Acetophenone4. BenzeneSampleVLDLEluent A2Reduced plate height *40˚CAcetophenoneBenzene3.23.62.42.3* Plate height / particle diameter of the packed resin4Eluent BComparison of ET-RP1’s column efficiencies (theoretical plate height) observed athigh and normal temperature conditionshigh temp. (150˚C)2.4mL/minnormal temp. (40˚C)0.5mL/min150˚CStandard AnalysisColumnsColumns for Chiral Separation Columns for High Temperature Reversed Phase Chromatography[Columns for Special Separation Modes] Column for Hydrophobic Interaction Chromatography Columns for Affinity ChromatographyColumn : Shodex ORpak CRX-853Eluent : 0.25mM CuSO 4 aq.Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CColumn : Shodex ET-RP1 4DEluent : (Left) H 2O/CH 3CN=50/50(Right) H 2O/CH 3CN=75/25Detector : Photodiode array(210nm)Column Oven : Polaratherm 9000 Series(SandraSelerity Technonogies, Inc)Note :The eluent was introduced into the columnafter being preheated and was cooled aftercolumn elution, then introduced into thedetector.Data provided by Research Institute for Chromatography bvba57