Shodex Catalogue 2013-2015.pdf - Analytics Shop

Barbital recovery rate comparison of ODP2 HP-2B and ODS in the presence of BSAODP2 HPBarbital 500ng/mLBarbitalPeak Area : 100%ODSBarbital 500ng/mLBarbitalPeak Area : 100%For the LC/MS analysis of drug samplescontaining protein as a matrix, using ODScolumns generally exhibit ion suppressionof the target drugs. Since proteins are notretained by ODP2 HP column, they eluteseparate (as a void) from the targetcomponents. Thus, using ODP2 HP columnexhibits less ion suppression influence.Standard AnalysisColumns0.0 1.0 2.0 3.0 4.0 min 0.00 2 4 6 8 100 1into MS2Barbital 500ng/mL in BSA 7.0mg/mL1BarbitalPeak Area : 99%LC/MS analysis of drugs in control serumSample : 5μLDrugs 500ng/mL each in Control serumUV(280m)12 minLC/MS analysis of basic drugs0Lidocaine2 4 6 8 10 12 14 min3 4 5 6 7 8 9 min21.0 2.0 3.0 4.0 minm/z 235Chlorpheniraminem/z 275VerapamilPropranololm/z 260Diphenhydraminem/z 256BupivacaineImipramineColumn : Shodex ODP2 HP-2BEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : UV(280nm), ESI-MS(SIM Positive)Column temp. : 40˚Cm/z 304 m/z 290m/z 289m/z 455m/z 281ODP-50 2D, a polymer-based reversed phase column, is capable of analyzingbasic drugs at low concentration or without salt. This is preferred for ESI-MSanalysis, since the presence of salt causes ion suppression, and thus influencesthe analysis performance.Column : Shodex Asahipak ODP-50 2DEluent : H 2 O/CH 3 CN=40/60Flow rate : 0.2mL/minDetector : ESI-MS(SIM Positive)Column temp. : 30˚CSample : 10ng/mL each, 5μL1. Scopolamine2. AtropineBarbital 500ng/mL in BSA 7.0mg/mLBarbitalPeak Area : 71%LC/MS analysis of statinsPravastatin0 1 2 3 4 5 6 7 8 9Glyoxylic acidPyruvic acidGlycolic acidLactic acidPitavastatinFluvastatinAtorvastatinMevastatin10 11 12 13 14 15 16 17 18 19 20 21 minMaleic acidLovastatinSuccinic acidFumaric acidSimvastatinLC/MS analysis of organic acidsColumn : Shodex ODP2 HP-2BODS from other manufacturerEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative : m/z 183)Column temp. : 30˚CInjection vol. : 10μLAdipic acid0 1 2 3 4 5 6 7 8 9 minSample : 100ng/mL each, 5μLm/z 423m/z 422m/z 412m/z 559m/z 391m/z 405m/z 419Column : Shodex ODP2 HP-2BEluent : (A); 10mM Ammonium formate aq. (B); CH 3 CNLinear gradient;B%; 10%(0min) 10%(3min) 35%(13min) 35%(20min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM)Column temp. : 40˚CSample : 50ng/mL each, 10μLm/z 73m/z 87m/z 75m/z 89m/z 115m/z 117m/z 115m/z 145Column : Shodex RSpak DE-213Eluent : (A); 0.1%(v/v) Formic acid aq. (B); CH 3 CNLinear gradient;B%; 5%(0min) 5%(2min) 15%(2.5min) 15%(10min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative)Column temp. : 30˚CColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography59