198 CUPRINS

amylovora type strain 1⁄79Sm did not produce uorescent pigment on King_s B
medium, formed yellow mucoid colonies on MM2Cu semi-selective medium and
produced levan on sucrose rich agar plates. In conclusion, the data obtained during
the course of this work for several isolates from pear and apple trees with re blight
symptoms from the central part of Republic of Moldova allow us to identify these
bacteria as E. amylovora.
100
Table 3. The biochemical (API 20 E identi cation system) characteristics of bacterial
isolates.
№ Morphological characteristics
1.
ONPG (2-nitrophenyl-ßD-galactopyranoside)
Isolate number
Bb1 Bb2 Bb3 Bb4
+ + + +
2. ADH (L-arginine) + + – +
3. LDC (L-lysine) – – – +
4. ODC (L-ornithine) – – – –
5. ICITI (trisodium citrate) – – – +
6. H 2 S (sodium triosulfate) – – – –
6. URE (urea) – – – –
7. TDA (L-tryptophane) – – – –
8. IND (L-tryptophane) – – – –
9. IVPI (sodium pyruvate) + + – +
10. IGELI (Gelatin (bovine origin)) – – – +
11. GLU (D-glucose) + + + +
12. MAN (D-mannitol) + + + +
13. INO (inositol) – – – –
14. SOR (D-sorbitol) – – – –
15. RHA (L-rhamnose) + + + +
16. SAC (D-sucrose) + + + +
17. MEL (D-melibiose) + + + +
18. AMY (amygdalin) + + + +
19. ARA (L-arabinose) + + + +
Acknowledgments
The research described in this publication was made possible in part by Award no.
MYSSP-1405 of the The Moldovan Research and Development Association (MRDA)
under funding from the US Civilian Research & Development Foundation (CRDF).in
agreement with the Academy of Sciences of Moldova (ASM).
References
1. Alsina, M., Blanch,
A. Improvement and update of a set of keys for biochemical identi cation of
Vibrio species. Journa of Applied Microbiology, Vol. 77, Nr. 6. 1994. P. 719-721.
2. Bazzi, C., Merighim, M., Zhang, Y., Jock, S., Geide,r K., López, M. Differentiation of Erwinia
amylovora strains in southern Europe by PFGE analysis. Acta Horticulturae: VIII International Workshop