209 CONTENTS

Buletinul AŞM. Ştiinţele vieţii. Nr. 2 (314) 2011
plant spacing. The middle 2 rows were harvested and the border rows were discarded,
and plot size was 9,66 m² at harvest. Trials were planted in April and were harvested
in September by hand.
To use in arti cial tests in growth chamber in the winter, broomrape seeds were
cleaned threshing the plants collected from different infested elds of Trakya Region.
Broomrape seeds were mixed with soil (sand, turf and normal soil) and mixture used
for screening in the pots (1-2 g / pots). Broomrape shoots were counted on the root
system of the sun ower plants of each pot after 45 days from planting. In the natural
infested elds in the Malkara, Tekirdag, Orobanche test plots were 5 m length, 14-15
plants existed each row with 35 cm x 1 m plant density in two replications. Susceptible
Sanbro hybrids existed as one control at each 40 rows.
Broomrape observations were evaluated as Frequency (F) Intensity (I) and Attacking
Rate (AR) based on Pustovoit method. The plants were accepted as resistant having %
0-10 Frequency and 0-1 AR values and [6; 11]. The plants had % 10-20 frequency as
accepted tolerant in the evaluations.
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F = % the number of plant with orobanche (The plant number infested
Orobanche / Total plants in the row x 100)
I = the number of orobanche in one infested plant (Total orobanche /
Total plants infested orobanche in the row).
AR = F x I / 100 = the number of orobanche in one plant in the row.
Results and discussion
Genetica, Biologia moleculară şi Ameliorarea
Broomrape results of the hybrids in the registration trials conducted in four locations
in Tekirdag province (Emiryakup and Buzagaci villages in Hayrabolu, and Ballihoca
and Inanlı villages in Muratli county) and one location in Edirne Province (Haskoy
village) were investigated in the study. Based on the Orobanche infestation of the
hybrids in the trials, sun ower hybrids exhibited different reactions against broomrape.
PR63F73, XF-4223 PR64H37 Pioneer Seed Co hybrids and LG5400 Limagrain Seed
Co hybrid showed resistance to new broomrape races in all location as staying less
than 10% frequency level. However, PR64H34 from Pioneer Seed Co, NX 53938 from
Syngenta Seed Co., 08-TR-003 from Trakya ARE and Tunca from Limagrain Seed Co.
exhibited also generally resistance but in some location they are found as tolerant with
staying at less than 20% frequency level (Table 1).
Broomrape resistant hybrids were given as bold in the Table 1. On the other hand,
LG5580 and LG5550 hybrids from Limagrain Seed Co, Reyna hybrid from May
Seed Co., Allioli hybrid from Akdeniz Seed Co., NX 78735 and DKF-2525 hybrid
from Syngenta Seed Co. selling as resistant or highly tolerant class but they exhibited
generally susceptibility in many locations except in a few locations. However, the
number of resistant and tolerant hybrids increases year by year in Turkey. Comparing
frequency level of sun ower hybrids, broomrapes revealed more aggressiveness in
Emiryakup in Hayrabolu and Inanli in Muratli than other three locations (Table 1).
Comparing of oil content, seed and oil yield of hybrids in the registration trials in
Trakya region as most natural infested areas, highest oil yields were obtained from
LG5580, 08-TR-003 and Tunca hybrids respectively (Table 2). On the other hand,