BuMa_2010_04 - Deutsche Bunsengesellschaft für Physikalische ...

PREISE/EHRUNGEN
156
POSTERPREISE
DER BUNSENTAGUNG 2010
BUNSEN-MAGAZIN · 12. JAHRGANG · 4/2010
Mit über 200 Postern präsentierte die Posterausstellung einen wesentlichen Teil des wissenschaftlichen Programms der Bunsentagung
2010. Damit die Poster noch sichtbarer werden, wurden durch ein Gutachtergremium nicht nur die drei Posterpreise ausgewählt,
sondern auch „hot Topic“ Poster. Alle ausgewählten Poster wurden in der Abschluss-Plenarsitzung von den Posterautoren
mit aussagekräftigen Folien vorgestellt.
POSTERPREISE
Posterpreisträger (von links) Konrad Meister, Wolfgang Christen, Sebastian
Weiße mir Erstem Vorsitzenden der Bunsen-Gesellschaft Wolfgang von
Rybinski (rechts).
P16
Metal-carbonyl complexes as a new modality for label-free live
cell imaging by Raman microspectroscopy
Konrad Meister, Johanna Niesel, Ulrich Schatzschneider, Nils
Metzler-Nolte, Diedrich A. Schmidt, Martina Havenith
With the help of confocal Raman microspectroscopy we were
able to localize the biological active metal compound [Mn(tpm)
(CO) 3]Cl in living cells. The goal of the collaboration between
the research groups of Professor Havenith and Professor Metzler-Nolte
within the Research Department of Interfacial Systems
Chemistry in Bochum is to provide new insights into the
mode of actions of metal-containing drugs.
3D Raman intensity images of HT29 colon cancer cells incubated
with an aqueous solution of [Mn(tpm(CO) 3]Cl show that this
compound penetrates the cell and selectively accumulates in
the nuclear membrane and the nucleolus. The C ≡ O stretching
vibration of the compound serves as an intrinsic spectroscopic
marker since the C≡O bond is in a region where no cellular
bands are present. This work shows that a label-free detection
of metal-carbonyls in living cells is possible. The localization of
the compound in the nucleus gives the synthetic chemists new
valuable hints on the biological mode of action and possible
improvements of the compound.
See e .g.: K. Meister, J. Niesel, U. Schatzschneider, N. Metzler-Nolte,
D. A. Schmidt, M. Havenith, “Label-Free Imaging of solvated Metal-
Carbonyl Complexes in Live Cells by Raman Microspectroscopy”,
Angewandte Chemie International Edition 49, 3310-3312 (2010)
P74
4D-Tracking of pathogens by Digital In-line Holographic Microscopy
Sebastian Weiße, Matthias Heydt, Niko Heddergott, Markus
Engstler, Michael Grunze, Axel Rosenhahn
The blood parasite Trypanosoma brucei is the causative agent
of the lethal African Sleeping Sickness. One possible mechanism
by which the parasite evades the host’s immune system
is based on the self-propulsion of the pathogen (Engstler et
al., Cell 2007). Since to date there is not known much about
the complex swimming patterns of Trypanosoma brucei we
investigate the organism‘s motility by using digital in-line holographic
microscopy (Xu et al., Opt Lett 2003; Heydt et al., Journal
of Adhesion 2007). This technique enables us to conduct
a fully three-dimensional and therefore quantitative motility
analysis. We developed a transportable holographic microscope
allowing measurements under the control of experimental
parameters such as temperature and viscosity under
fl ow or static conditions and therefore a systematic variation
of these factors.
Engstler, M. et al. „Hydrodynamic fl ow-mediated protein sorting
on the cell surface of trypanosomes.“ Cell 131, 505-515
(2007).
Heydt, M et al. „Digital in-line holography as a three-dimensional
tool to study motile marine organisms during their exploration
of surfaces.“ Journal of Adhesion 83, 417-430 (2007).
Xu, W. et al. „Tracking particles in four dimensions with in-line
holographic microscopy.“ Optics Letters 28, 164-166 (2003).