Mireille Consalvey PhD Thesis - University of St Andrews

Chqpiut 4 Lahorator\ ]m C. ý, tiuatioll illio Iniclooll (ohCrithic
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mi, ýIajjr
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lights (-650 ýLrnol M-2 s-1). Table 4.2. shows the times and exposure period for
each experiment.
Table 4.2. Experimental times
I Exposure period
Site Time Duration Low tide
Tay 08: 14 - 13: 35 337 min 10: 27 (133 min)
(June 1999)
Eden 08: 12 - 14: 59 407 min 11: 08 (176 min)
(February 2000)
Eden 10: 00 16: 30 1 390
- min 13: 10 (159 min)
(April 2000)
4.2.2. Lens tissue collection
Lens tissues were sprayed with salt water and placed on the core using a
standard pattern (Figure 4.3). This enabled all the lens tissues to be placed on
each core at the same time. A piece of lens tissue from each core was
immediately removed and placed in 4% gluteraldehyde solution. A piece of lens
tissue was then removed from each core approximately every hour over the low
tide period. Pen-nanent slides were prepared from the fixed samples.
4.2.3. Fluorescence measurements
Fluorescence measurements were taken approximately once an
I
hour. A
dark adaptation chamber (Figure 4.3) was placed on the exposed sediment of
each core and left for 15 min. The FMS2 portable fluorometer was used to
measure the parameters F0 15 and F 15 FF
et al. 1989).
.
(Honeywill et al. 2001) and /" (Genty
103
115