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Mireille Consalvey PhD Thesis - University of St Andrews

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Bi0fillill formation jild<br />

intervals Taylor and Paterson (1998) determined there to be a significant log<br />

linear decrease in the concentration <strong>of</strong> colloidal carbohydrates in the top 2 mm.<br />

Kelly et al. (2001) further characterised the depth distribution <strong>of</strong> chlorophyll a to<br />

determine a linear decrease with depth. Kelly et al. (2001) significantly<br />

positively correlated chlorophyll a and colloidal carbohydrate concentrations,<br />

finding a greater co-vanation in micro-sectioned samples.<br />

3.1.1. Aims<br />

The aims <strong>of</strong> this work were to determine the physical and biological<br />

changes associated with the growth and development <strong>of</strong> a microphytobenthic<br />

bi<strong>of</strong>ilm under in situ and controlled laboratory conditions. Two separate<br />

experiments were designed; the first was a simulated in situ disturbance event<br />

following the growth and recovery <strong>of</strong> a bi<strong>of</strong>ilm after the removal <strong>of</strong> the surface<br />

layer <strong>of</strong> sediment, and the second was a controlled laboratory study examining<br />

the growth and development <strong>of</strong> a bi<strong>of</strong>ilm using fine scale sectioning (200 ttm)<br />

techniques.<br />

3.2. Materials and Methods<br />

3.2.1. The Cohesive <strong>St</strong>rength Meter<br />

The Cohesive <strong>St</strong>rength Meter (CSM; Paterson 1989; Figure 3. IA) is a<br />

device that measures the erosion potential <strong>of</strong> sediments. The CSM consists <strong>of</strong> a<br />

water filled chamber which is inserted into the sediment (Figure 3.1B). A<br />

downward nozzle directs a vertical stream <strong>of</strong> water onto the sediment surface in<br />

short pulses (Figure 3.1C). The velocity <strong>of</strong> the water jet is systematically<br />

67

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