Mireille Consalvey PhD Thesis - University of St Andrews

Chapter 2...... Materials and Methods
Protocol 2:
This gradient was run for 40 min and used an additional Solvent, Solvent
C (Table 2.2; Figure 2.7) to aid carotene elution (BIOPTIS).
Solvents A and B: as above
Solvent C: 565 ml of methanol and 435ml of propanol.
Table 2.2. Protocol 2
Step Time
(min)
Time
(cumulative)
Flow rate
(ml min-1)
%A %B %C Curve
0 0 0 1 100 0 0
1 5 5 1 50 50 0 1
2 5 10 1 50 50 0
3 5 15 1 0 100 0 1
4 10 25 1 0 100 0
5 2 27 1 100 0 0 1
6 2 29 1 0 0 100 1
7 3 32 1 0 0 100
8 3 35 1 100 0 0 1
9 5 40 1 0 0
5 10 iý, 1.
Tirra(MrvlEs)
Figure 2.7. HPLC Gradient: Protocol 2.
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